The preparation method of gardenia blue pigment

Abstract

The invention discloses a preparation method of gardenia blue pigment. The specific process steps are as follows: (1) preparation of gardenia leaf medium, including preparation of solid medium and liquid medium; (2) preparation of liquid seeds of Aspergillus, including preparation of primary, secondary and tertiary seeds; (3) inoculation Fermentation, including liquid-solid two-way fermentation and liquid fermentation; (4) Enzymatic hydrolysis of gardeniside, including liquid-solid two-way fermentation and liquid fermentation; (5) Gardenia blue pigment generation and purification. The gardenia aglycone and amino acid are condensed by adding amino acid to form gardenia blue pigment, and the pigment is purified by resin column, and finally a blue-black powder is obtained, which is the gardenia blue pigment with a color value greater than 190 (E1%1cm 590nm). The invention utilizes the gardenia leaves to produce the gardenia blue pigment, which greatly reduces the production cost; the process of the invention is simple and stable, the investment in the production equipment can be large or small, and the production is flexible, and is suitable for the investment of enterprises of various scales.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation of biological products, and in particular relates to a preparation method of gardenia blue pigment. Background technique [0002] Gardenia blue pigment was initially produced by hydrolyzing geniposide in gardenia fruit and reacting with amino acids. Gardenia blue pigment is soluble in water and low-alcohol and other hydrophilic solvents. It has excellent physical and chemical properties such as light and heat resistance, acid and alkali resistance, and unique dyeing effect. Gardenia blue pigment can not only replace synthetic pigments by itself, but can also be used in harmony with red and yellow pigments of other strains, and can be blended into a series of blue-green hues such as green, purple, and brown. Gardenia blue pigment has been widely used in many fields, such as food and medicine, due to its natural source, safety, non-toxicity, good human compatibility, excellent ph...

AI Technical Summary

Problems solved by technology

Gardenia blue pigment has been widely used in many fields, such as food and medicine, due to its natural source, safety, non-toxicity, good human compatibility, excellent physical and chemical properties, and remarkable dyeing effect since its appearance. Increasingly, however, the traditional preparation method is to extract from gardenia fruit, and gardenia fruit contains a large amount of interfering substances, resulting in complex extraction process, excessive material consumption, low yield, and high raw material cost, the product Extremely expensive, which limits the large-scale application of such ingredients and the development of related industries in my country in recent years