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Sea cucumber-like nano composite material and preparation method and application thereof

A technology of nanocomposite materials and sea cucumbers, applied in nanotechnology, nanotechnology, material excitation analysis, etc., can solve problems such as poor stability, poor dispersion, and increased number of hot spots, and achieve increased hot spot distribution, Raman scattering, and improved efficiency and the effect of sensitivity

Active Publication Date: 2015-07-01
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The number of hot spots on a single nanomaterial of the nanocomposite material of the present invention has been greatly increased, which will greatly improve the efficiency of Raman detection using hot spots; at the same time, due to the use of a core-shell structure, the stability of the material is improved, effectively avoiding Solved the problems of general nanoparticle stability and poor dispersion

Method used

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  • Sea cucumber-like nano composite material and preparation method and application thereof
  • Sea cucumber-like nano composite material and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0057] Gold nanorods (Chem Mater, 2003, 15(10): 1957-62.) were prepared by regulating the concentration of silver nitrate by using hexadecyltrimethylammonium bromide as a surfactant by seed-induced growth method, and using the Gold nanorods prepare sea cucumber-like nanocomposites, the specific method is as follows:

[0058] (1) Preparation of gold nanorods, and peptide nucleic acid modification of gold nanorods:

[0059] a) Synthesis of seed crystals: Add 7.5 mL of 100 mM cetyltrimethylammonium bromide to a 25 mL round bottom flask, add 50 μL of 2% (w / v) chloroauric acid; quickly add 600 μL of 10 mM Pre-cool sodium borohydride and stir evenly; when the color of the solution changes from colorless to brown, stop stirring, and stand at 30°C for 2 hours for later use;

[0060] b) Growth of gold nanorods: Add 10 mL of 100 mM cetyltrimethylammonium bromide and 80 μL of 2% (w / v) chloroauric acid in a 25 mL round bottom flask; add 75 μL of 10 mM nitric acid under stirring condition...

Embodiment 2

[0070] The gold nanorods were prepared by the seed crystal induced growth method, and the sea cucumber-like nanocomposites were prepared by using the gold nanorods, the specific method is as follows:

[0071] (1) Preparation of gold nanorods, and peptide nucleic acid modification of gold nanorods:

[0072] Add methionine, Cy3 (3H-indocyanine dye) modified PNA (19 monomers long, 3μM) into 5mM Tris-hydrochloride buffer, add gold nanorods (10nM, gold nano Rods: PNA=1:400) were placed in a warm water bath at 28°C for modification; after modification overnight, the gold nanorods modified with PNA were centrifuged (10000rpm, 30min) and washed 3 times with 5mM Tris-hydrochloric acid buffer to remove excess PNA; The final concentration was detected by UV-Vis spectrophotometer.

[0073] (2) Silver layer coated on the surface of the gold nanorods obtained in step (1)

[0074] Take 100 μL of 2nM PNA-modified gold nanorods, add 2 μL of cetyltrimethylammonium chloride with a mass concent...

Embodiment 3

[0078] The gold nanorods were prepared by the seed crystal induced growth method, and the sea cucumber-like nanocomposites were prepared by using the gold nanorods, the specific method is as follows:

[0079] (1) Preparation of gold nanorods, and peptide nucleic acid modification of gold nanorods:

[0080] Add cysteine, Cy5 (3H-indocyanine dye) modified PNA (80 monomers long, 3μM) into 5mM acetic acid-sodium acetate buffer, and add gold nanorods (10nM, gold Nanorods: PNA=1:500) were modified in a warm water bath at 32°C; after modification overnight, the gold nanorods modified with PNA were centrifuged (9000rpm, 40min) and washed 3 times with 5mM acetic acid-sodium acetate buffer to remove excess PNA; the modified concentration was detected by a UV-Vis spectrophotometer.

[0081] (2) Silver layer coated on the surface of the gold nanorods obtained in step (1)

[0082] Take 100 μL of 2nM PNA-modified gold nanorods, add 2 μL of sodium dodecyl sulfate with a mass concentration ...

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Abstract

The invention relates to a sea cucumber-like nano composite material and a preparation method and application thereof. The sea cucumber-like nano composite material is in a core-shell structure and comprises a peptide nucleic acid-modified gold nanorod, a nano silver layer and gold nanoparticles. The preparation method of the sea cucumber-like nano composite material comprises taking the PNA-modified (peptide nucleic acid-modified) gold nanorod as the core, coating the surface of the gold nanorod with silver and growing the gold nanoparticles on the surface of the silver to obtain the sea cucumber-like core-shell structure. The sea cucumber-shaped nano composite material contains a large number of hot spots for plasma resonance which are distributed on the surface of the gold nanorod, thereby greatly improving the efficiency of Raman detection through the hot spots. The sea cucumber-shaped nano composite material is simple in synthesis and stable in properties and can be applied to high-sensitivity Raman detection.

Description

technical field [0001] The invention relates to the field of nanocomposite materials, in particular to a nanocomposite material and its preparation method and application, in particular to a sea cucumber-like nanocomposite material and its preparation method and application. Background technique [0002] At present, people urgently need detection tools with high sensitivity and portability to deal with current threats in early diagnosis, environmental monitoring, pesticide residues, explosives, etc. The development of nanotechnology provides a technical basis for these detection techniques. Among them, the surface Raman-enhanced scattering technology developed by using noble metal nanoplasmons is one of the effective methods to solve this problem. [0003] In the research of surface-enhanced Raman scattering technology, an important phenomenon is found, that is, the "hot spot" phenomenon. "Hot spots" are due to superposition of plasmon resonances at certain locations on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F1/02G01N21/65B82Y30/00
Inventor 丁宝全邹翊国刘欢王振刚苏晓芳蒋乔
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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