Super-green seawater pearl cultivation method
A cultivation method, emerald green technology, applied in the field of cultivation of emerald green seawater pearls, can solve problems such as unapplied pearl cultivation technology, and achieve the effect of increasing pearl yield, avoiding damage, and improving survival rate
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Embodiment 1
[0043] The cultivation method of the emerald green seawater pearl of the present embodiment, comprises the following steps:
[0044] (1) Preparation of chromium-containing nutrient solution: Use a microporous membrane with a pore size of 0.22 μm to filter seawater as a solvent for the preparation of CrCl 2 ·6H 2 O's modified f / 2 medium to obtain chromium-containing nutrient solution.
[0045] The chromium-containing nutrient solution uses the above-mentioned filtered seawater as a solvent, and is added with:
[0046]
[0047] (2), preparation of chromium-rich diatom mud: the diatoms in the logarithmic growth phase, when OD440nm is 0.15, inoculated in the chromium-containing nutrient solution and cultivated continuously for 7 days to obtain diatom mud; ℃, 4000rpm, refrigerated and centrifuged for 20 minutes to retain the precipitate; then rinse the precipitate with deionized water 3 times to obtain chromium-rich diatom mud.
[0048] (3) Feeding Pinctada martensii: choose ...
Embodiment 2
[0052] The cultivation method of the emerald green seawater pearl of the present embodiment, comprises the following steps:
[0053] (1) Preparation of chromium-containing nutrient solution: use a microporous membrane with a pore size of 0.22 μm to filter seawater as a solvent for the preparation of CrSO 4 ·6H 2 O's modified f / 2 medium to obtain chromium-containing nutrient solution.
[0054] The chromium-containing nutrient solution uses the above-mentioned filtered seawater as a solvent, and is added with:
[0055]
[0056] (2), preparation of chromium-rich diatom mud: Diatom mud in the logarithmic growth phase, when OD440nm is 0.45, inoculated in chromium-containing nutrient solution and cultured continuously for 7 days to obtain diatom mud; diatom mud at 4 ° C , 3500rpm, high-speed refrigerated centrifugation for 30min, retain the precipitate, and then rinse the precipitate with deionized water 5 times to obtain chromium-rich diatom mud;
[0057] (3) Feeding Pinctada...
Embodiment 3
[0061] The cultivation method of the emerald green seawater pearl of the present embodiment, comprises the following steps:
[0062] (1) Preparation of chromium-containing nutrient solution: use a microporous membrane with a pore size of 0.45 μm to filter seawater as a solvent for the preparation of Cr(NO 3 ) 2 9H 2 O's modified f / 2 medium to obtain chromium-containing nutrient solution.
[0063] The chromium-containing nutrient solution uses the above-mentioned filtered seawater as a solvent, and is added with:
[0064]
[0065] (2), preparation of chromium-rich spirulina mud: the spirulina in the logarithmic growth phase, when OD440nm is 0.45, inoculated in the chromium-containing nutrient solution and cultured continuously for 7 days, to obtain the spirulina mud; put the spirulina mud at 0 ℃ , 4000rpm, high-speed refrigerated centrifugation for 30min, retain the precipitate; then wash the precipitate with deionized water 3 times to obtain chromium-rich spirulina mud. ...
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