Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Thsd7a gene sequence and expression change detection and its application in the prediction of coronary heart disease

一种DNA序列、冠心病的技术，应用在THSD7A基因序列及表达改变检测及其在冠心病预测中的应用领域，能够解决易感基因缺乏有效的方法、不足以解释冠心病遗传基础等问题，达到降低发病率的效果

Active Publication Date: 2018-03-27
田小利 +1
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, only 6 of the discovered loci are based on the research findings of the Chinese Han population, which is far from enough to explain the genetic basis of coronary heart disease in the Chinese Han population
In addition, most of the existing studies only focus on the related loci, and there is no effective method for identifying the susceptibility genes referred to by the related loci.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Thsd7a gene sequence and expression change detection and its application in the prediction of coronary heart disease
  • Thsd7a gene sequence and expression change detection and its application in the prediction of coronary heart disease
  • Thsd7a gene sequence and expression change detection and its application in the prediction of coronary heart disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1: Genome-wide screening of coronary heart disease susceptibility loci:

[0070] Experimental population

[0071] In this example, the patients with coronary heart disease (coronary angiography positive), that is, the samples and case data of the coronary heart disease case group came from the General Hospital of the Chinese People's Liberation Army, and the samples and data of the control group came from the Beijing Physical Examination Center. All samples from the population participating in the experiment were notified in writing, and informed consent was signed by themselves or their family members. The experimental operation followed the ethical principles stipulated in the "Declaration of Helsinki" and was reviewed and approved by the Peking University Ethics Committee.

[0072] Among them, the selection criteria for samples from the coronary heart disease case group meet one of the following three conditions:

[0073] (1) Previous medical history of myo...

Embodiment 2

[0299] Multi-center verification of embodiment 2 rs17165136 locus

[0300] In order to further verify the rs17165136 single nucleotide polymorphism site in Example 1, this example will carry out multi-center verification on the above site. The method used for verification is still the combination of LCR and direct sequencing. The specific operation is as follows:

[0301] research population

[0302] The samples and data of patients with coronary heart disease (positive coronary angiography) used in the experiment came from the Affiliated Hospital of Harbin Medical University, China-Japan Friendship Hospital, Fuwai Hospital, and the First Affiliated Hospital of Zhengzhou University. The control samples and data came from the physical examination centers in Northeast and North regions that matched the regions of the cases; the case samples and data verified by the southern population came from the First People's Hospital of Jiangsu Province, the First People's Hospital of Nin...

Embodiment 3

[0315] Example 3 Identification of susceptibility genes

[0316] Unlike many SNP sites found in GWAS located in gene deserts, the rs17165136 site identified in this application is located in the THSD7A intron region, so the inventors hypothesized that the THSD7A gene may be a susceptibility gene for coronary heart disease. And verify it through this embodiment.

[0317] research population

[0318] The population used for eQTL analysis of THSD7A gene came from the Beijing Physical Examination Center. All samples from the population participating in the experiment were notified in writing, and informed consent was signed by themselves or their family members. The experimental operation followed the ethical principles stipulated in the "Declaration of Helsinki" and was reviewed and approved by the Peking University Ethics Committee.

[0319] cell culture

[0320]Human embryonic kidney cell line HEK293A was preserved by this experiment; primary human umbilical vein endothelia...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biology. On one hand, the invention relates to a single nucleotide polymorphism (SNP) site of a coronary heart disease susceptible gene THSD7A (Thrombospondin type-1 domain-containing 7A) and a corresponding composition and a corresponding kit which are used for detecting the SNP site; the invention especially relates to application of a nucleic acid affinity ligand of the SNP site to preparation of a composition which is used for detecting, screening or predicting coronary heart disease susceptibility of Chinese Han population. On the other hand, the invention relates to a corresponding composition and a corresponding kit which are used for detecting the THSD7A gene and further relates to application of a nucleic acid affinity ligand of the THSD7A gene to preparation of the composition for detecting, screening or predicting the coronary heart disease susceptibility of Chinese Han population. The invention provides new further scientific evidence for researching and developing targeted coronary heart disease gene therapy, and a new clue is provided for finding a new coronary heart disease molecule mechanism and developing a new medicine target point and individualized treatment of a coronary heart disease.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a single nucleotide polymorphism (single nucleotide polymorphism, SNP) site on the THSD7A gene associated with susceptibility to coronary heart disease, and a corresponding combination for detecting the SNP site The invention relates to a substance and a kit, in particular to the application of the nucleic acid affinity ligand of the SNP site in the preparation of a composition for detecting, screening or predicting the susceptibility of coronary heart disease in Han population. Background technique [0002] Coronary heart disease, also known as coronary heart disease, is an ischemic heart disease caused by coronary artery wall atherosclerosis with morphological changes of coronary artery stenosis or even occlusion. Coronary heart disease is extremely harmful. According to the data of the World Health Organization, ischemic heart disease, mainly coronary heart disease, is the number ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6883
CPCC12Q1/6883C12Q2600/156
Inventor 田小利李扬
Owner 田小利
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com