A three-dimensional printing method and device for integrated manufacturing of tissue/organ chips

Abstract

The invention relates to a three-dimensional printing method and device for tissue / organ chip integrated manufacturing. The three-dimensional printing method comprises the following steps: (1), designing the three-dimensional structural drawing of a three-dimensional chip, and converting the three-dimensional structural drawing into a slice layer graphic file format; (2), starting a three-dimensional printing device, and absorbing printing ink into all spraying heads; leading in slice layer graphic files; (3),respectively printing a main body material printing ink, a sacrificial material printing ink and different cell printing ink into pre-designed positions of a bottom plate system through the three-dimensional printing device; (4), repeating the step (3) and accomplish printing of a three-dimensional chip structure layer by layer and in an accumulating manner until the printing to a slice layer graphic file is finished; (5), heating or refrigerating the three-dimensional chip integrally printed, so as to enable a channel sacrificial material to be changed into a sol state; (6), using a pipette to absorb the channel sacrificial material in the sol state, removing the channel sacrificial material, and forming an integrated three-dimensional chip structure; (7), carrying out crosslinking on the cell printing ink material not molten, and carrying out culture medium perfusion.

Description

technical field [0001] The invention relates to the field of three-dimensional bioprinting, in particular to a three-dimensional printing method and device for tissue / organ chip integrated manufacturing. Background technique [0002] The extracellular microenvironment plays an important role in the formation and development of tissues and organs, as well as the expression of cell behavior and function. At this stage, researchers generally use two-dimensional monolayer cell culture models or animal models to study and understand human tissue physiology and pathology. Because the two-dimensional monolayer cell culture model lacks the interaction between cells and cells and between cells and the matrix, and the immune system and cellular microenvironment of animal models are different from those of the human body, neither of them can accurately express the microenvironment of the human body. characteristics of the environment. At present, the existing three-dimensional models...

AI Technical Summary

Problems solved by technology

[0004] At this stage, researchers mainly use soft lithography technology to manufacture organ chips. This technology is difficult to realize the manufacture of organ chips with complex three-dimensional structures, and this technology relies on masks. The manufacturing cost of masks is high and the processing cycle is long, which reduces the cost. System flexibility

The current maskless organ chip manufacturing technologies include stereolithography technology, DMD (digital micromirror device) technology, Print and Peel technology (printing and peeling technology), etc. These technologies are not only difficult to realize organ chip manufacturing with complex multi-layer structures, Moreover, it is difficult to realize the controllable spatial distribution of cells in the organ chip, and the material of the extracellular matrix is ​​single

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