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Extraction and purification method of total alkaloids of Sophora sophora

A purification method, the technology of Sophora sophora, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of organic solvent residues, unfavorable clean production, low extraction rate, etc., and reach the production cycle Short, fast adsorption speed, large adsorption effect

Active Publication Date: 2019-08-02
NINGXIA VOCATIONAL TECH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction rates of the above two methods are relatively low, 2.1% and 2.3% respectively. At the same time, organic solvents are used for separation and purification, which is not conducive to clean production, and the product has the disadvantage of residual organic solvents

Method used

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  • Extraction and purification method of total alkaloids of Sophora sophora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Take 100kg of Sophora sophora as an example.

[0023] 1. Cutting

[0024] Cut the weighed Alopecia sophora into small pieces of 1-2 cm, and set aside.

[0025] 2. Cellulase enzymatic hydrolysis

[0026] Put the sliced ​​Alopecia sophora into a multi-functional extraction tank (note that the feeding should be even and tight, so as to facilitate the cooking and extraction in the subsequent process), add water (the ratio of material to liquid is 1:8), adjust the pH to 5 with hydrochloric acid, and add its weight 0.1% cellulase, and incubated at 50°C for 4h.

[0027] 3. Extract after decoction and extraction

[0028] Cover the multi-functional extraction tank containing the material after enzymatic hydrolysis, add hydrochloric acid until the pH value of the liquid is 3, heat to a slight boil on the liquid surface, discharge the material after 2 hours, put the filtrate into the liquid storage tank, and then extract Add dilute hydrochloric acid solution (pH ...

Embodiment 2

[0042] Embodiment 2: Take 100kg of Sophora sophora as an example.

[0043] 1. Cutting

[0044] Cut the weighed Alopecia sophora into small pieces of 1-2 cm, and set aside.

[0045] 2. Cellulase enzymatic hydrolysis

[0046] Put the sliced ​​Alopecia sophora into a multi-functional extraction tank (note that the feeding should be tight and even, so as to facilitate the cooking and extraction in the subsequent process), add water (the ratio of material to liquid is 1:10), adjust the pH to 4 with hydrochloric acid, and add its weight 0.2% cellulase, and incubated at 35°C for 4h.

[0047] 3. Extract after decoction and extraction

[0048] Cover the multi-functional extraction tank containing the material after enzymatic hydrolysis, add hydrochloric acid until the pH value of the liquid is 2, heat until the liquid surface boils slightly, discharge the material after 2 hours, put the filtrate into the liquid storage tank, and then extract Add dilute hydrochloric acid solution (p...

Embodiment 3

[0062] Embodiment 3: Take 200kg of Sophora Sophorae as an example.

[0063] 1. Cutting

[0064] Cut the weighed Alopecia sophora into small pieces of 1-2 cm, and set aside.

[0065] 2. Cellulase enzymatic hydrolysis

[0066] Put the sliced ​​Alopecia sophora into a multi-functional extraction tank (note that the feeding should be tight and even, so as to facilitate the cooking and extraction in the subsequent process), add water (the ratio of material to liquid is 1:9), adjust the pH to 6 with hydrochloric acid, and add its weight 0.3% cellulase, and incubated at 65°C for 6h.

[0067] 3. Extract after decoction and extraction

[0068] Cover the multi-functional extraction tank containing the material after enzymatic hydrolysis, add hydrochloric acid until the pH value of the liquid is 4, heat to a slight boil on the liquid surface, discharge the material after 2 hours, put the filtrate into the liquid storage tank, and then extract Add dilute hydrochloric acid solution (pH...

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Abstract

The invention relates to a method for extracting and purifying total alkali of sophora alopecuroides. The method comprises the following processing steps: carrying out enzymolysis on sophora alopecuroides with cellulose, decocting, extracting and filtering; carrying out vacuum concentration on the obtained filtrate until the relative density is 1.0-1.1, carrying out alcohol precipitation and centrifugal separation, carrying out vacuum concentration on the obtained filtrate until the relative density is 1.0-1.1; standing clear paste when the pH is 8-9, and centrifugally separating; adsorbing and extracting the obtained supernate with macroporous resin, carrying out vacuum concentration on the collected eluent until the relative density is 1.05-1.15, and carrying out spray drying. The cellulose is applied to auxiliary extraction of total alkali of sophora alopecuroides; a sophora alopecuroides extract liquid is separated and purified by a macroporous resin adsorption process; and finally, the extraction rate is improved to 3.7% from 2.3%, so that the method is short in extraction time, low in energy consumption, free of pollution, and suitable for industrialized production; and industrial continuous production can be realized.

Description

technical field [0001] The invention relates to the technical field of extraction and separation of traditional Chinese medicines, in particular to an extraction and purification method of total alkaloids of Sophora sophora. Background technique [0002] Sophora aLopecuroides L. is a plant of the genus Sophora in the family Fabaceae, and is used for medicinal purposes with the whole plant, seeds and rhizomes. Cold in nature, bitter in taste, poisonous. The function of the whole herb is to clear away heat and detoxify, stop dysentery. The total alkaloids of Sophora sophora and various monomeric alkaloids can be extracted from the whole herb of Sophora sophora, and the extraction of total alkaloids of sophora sophora as raw material has formed an industrialization. The traditional extraction of total alkaloids of Sophora alopecia is mainly cold soaking and reflux extraction. The cold soaking method is to use dilute hydrochloric acid solution with pH=2-4 as solvent, extract ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/489
Inventor 冷晓红郭鸿雁王秀芬李军郝彩琴
Owner NINGXIA VOCATIONAL TECH COLLEGE
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