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Aeromonas hydrophila and aeromonas veronii duplex oral sustained-release microsphere vaccine and preparation method thereof

A technology of Aeromonas victorii and Aeromonas hydrophila, applied in antibacterial drugs, microcapsules, pharmaceutical formulations, etc., can solve the problems of poor immune effect, achieve safe reagents, good immune effect, and promising application prospects broad effect

Active Publication Date: 2015-06-10
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the microcapsule antigen delivery system was applied to Aeromonas hydrophila and Aeromonas victoria, and a dual microcapsule oral vaccine of Aeromonas hydrophila and Aeromonas victoria was developed to overcome the inactivation The defect of poor direct oral immune effect of vaccine

Method used

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  • Aeromonas hydrophila and aeromonas veronii duplex oral sustained-release microsphere vaccine and preparation method thereof
  • Aeromonas hydrophila and aeromonas veronii duplex oral sustained-release microsphere vaccine and preparation method thereof
  • Aeromonas hydrophila and aeromonas veronii duplex oral sustained-release microsphere vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] 1 material

[0035] 1.1 Strains

[0036] Aeromonas hydrophila and Aeromonas victoria strains were isolated from diseased fish suffering from bacterial septicemia in Xinxiang City, including 32 strains of Aeromonas hydrophila and 20 strains of Aeromonas vickeris, frozen Store dry.

[0037] 1.2 Test animals

[0038] Healthy crucian carp was purchased from a fishing ground in the suburbs of Xinxiang City. The weight of each fish was about 30g and the length was 12-15cm. Before use, domesticate them in the laboratory for 1 week, inflate them, control the water temperature at about 28°C, clean and change water, and feed them regularly.

[0039] 1.3 Medium

[0040] Preparation of LB nutrient broth: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, potassium dihydrogen phosphate 1.0g, distilled water 1000mL, mix well, heat to dissolve, adjust the pH value to 7.6, subpackage, and extinguish under high pressure at 112kPa bacteria for 15 minutes.

[0041] Preparation ...

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Abstract

The invention provides an aeromonas hydrophila and aeromonas veronii duplex oral sustained-release microsphere vaccine and a preparation method thereof, and belongs to the field of biological pharmacy. With sodium alga acid as a wall material, and inactivated whole bacteria of aeromonas hydrophila and aeromonas veronii as a core material, the aeromonas hydrophila and aeromonas veronii duplex oral sustained-release microsphere vaccine is prepared by an emulsification method. Prussian carp is immunized by the aeromonas hydrophila and aeromonas veronii-calcium alginate microcapsule oral vaccine, so that the serum enzyme activity and the leukocyte phagocytic activity can be significantly improved; the relative protection rate for the prussian carp is 46.7%; the microcapsule is stable in a simulated gastrointestinal condition; relatively good sudden release and slow release are displayed; the bacteria antigenicity in the microcapsule is kept well; and the vaccine safety and the storage stability are relatively good.

Description

technical field [0001] The invention relates to animal vaccine preparation technology, in particular to a dual oral sustained-release microsphere vaccine of Aeromonas hydrophila and Aeromonas victoria and a preparation method thereof. The vaccine of the present invention is mainly suitable for fish. Background technique [0002] Aeromonas hydrophil (Ah) and Aeromonas veronii (Av) can cause outbreaks of hemorrhagic sepsis in fish, amphibians, and reptiles, leading to skin bleeding in freshwater fish (hemorrhagic sepsis ) and a large number of deaths have brought huge economic losses to the large-scale freshwater fish farming industry. The application of traditional therapies such as antibiotics can easily induce a series of problems such as bacterial resistance, drug residues, and environmental ecological damage. [0003] The developed Aeromonas hydrophila vaccines (whole bacterium vaccines, genetically engineered vaccines) are all injection immunization or immersion immuni...

Claims

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Application Information

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IPC IPC(8): A61K39/106A61K47/36A61K9/50A61P31/04
Inventor 王文锋关建义侯玉娟吴佳静张敬梅邱海洋
Owner XINXIANG MEDICAL UNIV
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