High spatial resolution laser split pupil confocal spectroscopy-mass spectrometry microscopic imaging method and device

Abstract

The invention relates to a high-spatial-resolution laser spectral-pupil confocal spectrum-mass spectrum microscopic imaging method and device and belongs to the field of a confocal microscopic imaging technology, a spectral imaging technology and a mass spectrum imaging technology. The spectral-pupil confocal imaging technology, the mass spectrum imaging technology and a spectrum detection technology are combined; the small focused spot of a spectral-pupil confocal system processed by a super resolution technique is utilized to carry out high-spatial-resolution morphological imaging for a sample; a mass spectrum system is utilized to carry out micro area mass spectrum imaging for charged molecules and atoms generated by desorbing and ionizing the sample by the focused spot of the spectral-pupil confocal system; a spectrum detection system is utilized to carry out spectral imaging for the emission spectrum information of plasmas generated by desorbing and ionizing the sample by the focused spot of the spectral-pupil confocal system; then the imaging and detection of high-spatial-resolution and high sensitive morphology and compositions of a micro area of the sample are realized through fusion and comparison of detection data information. The method and the device can provide a new effective technical way for biomass spectrum high-resolution imaging.

Description

technical field [0001] The invention belongs to the field of confocal microscopic imaging technology, spectral imaging technology and mass spectrometry imaging technology, and combines split-pupil confocal microscopic imaging technology, mass spectrometry imaging technology and spectral detection technology, and relates to a laser split-pupil confocal spectrum-mass spectrometer with high spatial resolution. The micro-imaging method and device can be used for high-resolution imaging of biological mass spectrometry. [0002] technical background [0003] Mass Spectrometry (Mass Spectrometry) is to ionize the components in the sample, so that the generated charged atoms, molecules or molecular fragments with different charge-to-mass ratios are respectively focused under the action of an electric field and a magnetic field to obtain a sequence of mass-to-charge ratios. Spectrograph instrument. Mass spectrometry imaging is to perform mass spectrometry analysis on multiple small a...

AI Technical Summary

Problems solved by technology

[0006] 1) Due to the use of simple laser focusing to desorb and ionize the sample, there are still problems such as large laser focusing spot and low spatial resolution of mass spectrometry detection;

[0007] 2) It is impossible to detect neutral atoms, molecules, neutral ions and groups, etc., which restricts the accurate and complete acquisition of the mass spectrum components of the sample;

[0008] 3) The time required for mass spectrometry imaging is long, and the axial position of the focused spot of the laser mass spectrometer often drifts relative to the sample to be measured

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