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Yeast culture and preparation method thereof

A technology of yeast culture and yeast, which is applied in animal feed, animal feed, application, etc., can solve the problems of low number of viable bacteria, low fermentation effect, and inability of yeast to fully utilize solid fermentation raw materials, so as to achieve rich enzyme system and high yield Strong enzyme effect

Active Publication Date: 2015-06-03
北京英惠尔生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the commonly used solid fermentation method of yeast culture has the following problems: the number of viable bacteria cultured is low, and the yeast cannot make full use of the solid fermentation raw materials, resulting in low fermentation effect and high economic cost, which cannot meet people's requirements

Method used

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  • Yeast culture and preparation method thereof
  • Yeast culture and preparation method thereof
  • Yeast culture and preparation method thereof

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Embodiment 1

[0027] As an embodiment of the present invention, the preparation method of the yeast culture comprises the following steps:

[0028] (1) Activate the strain of Saccharomyces cerevisiae, then inoculate the isolated and purified Saccharomyces cerevisiae into the primary shake flask culture medium, the medium capacity of 500ml Erlenmeyer flask is 100ml, shake the flask at 28~30℃, 180~200rpm Cultivate for 17-24 hours to obtain the primary seed liquid, then inoculate the primary seed liquid into the secondary shake flask culture medium, fill a 2000ml Erlenmeyer flask with a volume of 450ml, and culture the shake flask at 28°C-30°C and 180-200rpm for 20-24h. Wherein, the seed medium of the yeast is YEPD liquid medium, and the composition of the YEPD liquid medium is: glucose 20g, peptone 20g, yeast powder 10g, 1000ml water, natural pH. The medium was sterilized at 115° C. for 30 minutes at high temperature.

[0029] In this embodiment, the Saccharomyces cerevisiae is Saccharomyces...

Embodiment 2

[0040] As an embodiment of the present invention, the preparation method of the yeast culture comprises the following steps:

[0041] (1) Activate the strain of Saccharomyces cerevisiae, then inoculate the isolated and purified Saccharomyces cerevisiae into the primary shake flask culture medium, the medium capacity of 500ml Erlenmeyer flask is 100ml, shake the flask at 28~30℃, 180~200rpm Cultivate for 17-24 hours to obtain the primary seed liquid, then inoculate the primary seed liquid into the secondary shake flask culture medium, fill a 2000ml Erlenmeyer flask with a volume of 450ml, and culture the shake flask at 28°C-30°C and 180-200rpm for 20-24h. Wherein, the seed medium of the yeast is YEPD liquid medium, and the composition of the YEPD liquid medium is: glucose 25g, peptone 20g, yeast powder 12g, 1000ml water, natural pH. The medium was sterilized at 115° C. for 30 minutes at high temperature.

[0042] In this embodiment, the Saccharomyces cerevisiae is Saccharomyces...

Embodiment 3

[0053] As an embodiment of the present invention, the preparation method of the yeast culture comprises the following steps:

[0054] (1) Activate the strain of Saccharomyces cerevisiae, then inoculate the isolated and purified Saccharomyces cerevisiae into the primary shake flask culture medium, the medium capacity of 500ml Erlenmeyer flask is 100ml, shake the flask at 28~30℃, 180~200rpm Cultivate for 17-24 hours to obtain the primary seed liquid, then inoculate the primary seed liquid into the secondary shake flask culture medium, fill a 2000ml Erlenmeyer flask with a volume of 450ml, and culture the shake flask at 28°C-30°C and 180-200rpm for 20-24h. Wherein, the seed medium of the yeast is YEPD liquid medium, and the composition of the YEPD liquid medium is: glucose 18g, peptone 22g, yeast powder 10g, 1000ml water, natural pH. The medium was sterilized at 115° C. for 30 minutes at high temperature.

[0055] In this embodiment, the Saccharomyces cerevisiae is Saccharomyces...

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Abstract

The invention discloses a yeast culture and a preparation method thereof. The preparation method comprises the following steps: mixing saccharomycetes liquid zymophyte liquid with a solid culture medium inoculated with aspergillus niger, and carrying out aerobic fermentation at 28-35 DEG C for 12-24 hours, so as to obtain a solid fermentation product; and carrying out anaerobic fermentation on the solid fermentation product at 28-35 DEG C for 48-85 hours, and drying an anaerobic fermentation product, so as to obtain the yeast culture. The yeast culture prepared by virtue the preparation method has special aroma; after the yeast culture is subjected to wall breaking by drying, benefiting components such as yeast cell walls and contents can take efficacies of food calling, stress resistance, intestinal tract benefiting and the like, which cannot be presented or embodied fully by live bacteria.

Description

technical field [0001] The invention relates to the technical field of solid-state fermentation of yeast cells, in particular to a yeast culture and a preparation method thereof. Background technique [0002] Yeast culture is a kind of microecological preparation, which refers to the microecological product formed after sufficient anaerobic fermentation on a specific medium under the control of specific process conditions. The micro-ecological product does not contain a large amount of active yeast cells, and the yeast only performs fermentation, and its survival has no effect on the use effect of the product. Its main active ingredients are extracellular metabolites produced by yeast using solid substrate fermentation, solid substrate denatured after fermentation, yeast cell content and yeast cell wall, etc. The composition of this product is complex, mainly including small peptides, organic acids, vitamins, flavor-enhancing substances, mannan oligosaccharides, β-glucans, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/16
Inventor 李慧梁廷银王宏梁秒
Owner 北京英惠尔生物技术有限公司
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