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A method of inhibiting the growth of weeds in paddy fields by using rice straw powder and sticky bacteria

A technology of myxobacteria and rice straw, applied in the biological field, can solve the problems of aggravation, serious hidden dangers of food safety, destruction of biodiversity, etc., and achieve the effects of abundant sources, remarkable weed suppression effect and simple preparation method.

Inactive Publication Date: 2017-05-10
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this unrestricted large-scale application of chemical herbicides has brought great hidden dangers to the ecological environment, resulting in serious pollution problems, increasing resistance of farmland weeds to herbicides, and herbicide residues in agricultural products. Negative impacts such as reports of exceeding the standard continue to spread and become more and more prominent
Among them, the increasing resistance of farmland weeds to herbicides makes the total amount of herbicides used in the agricultural cultivation management process more and more high, and the development of highly toxic herbicides is also increasing, resulting in vicious environmental damage The problem is difficult to recover in the short term; the pollution of the pedosphere, hydrosphere and even the atmosphere is also increasing, biodiversity is also being destroyed, food safety hazards are becoming more and more serious, and ultimately seriously affect the survival and health of human beings

Method used

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  • A method of inhibiting the growth of weeds in paddy fields by using rice straw powder and sticky bacteria
  • A method of inhibiting the growth of weeds in paddy fields by using rice straw powder and sticky bacteria

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Embodiment 1

[0026] The specific steps of the method for suppressing weeds in paddy fields by using rice straw powder to add sticky bacteria of the present invention are as follows:

[0027] (1) Raw materials: rice PI312777 dry rice stalks, paddy field soil, from Fuzhou, Fujian (teaching farm of Fujian Agriculture and Forestry University);

[0028] (2) Preparation of rice straw powder: 120 g of PI312777 dry rice straw was taken, crushed and passed through a 40-mesh sieve to obtain PI312777 rice straw powder.

[0029] (3) Isolation of myxobacteria: Take 10g of paddy field soil, spread it evenly in a petri dish after natural air drying, and use 40mg / L gentamycin, 10mg / L kanamycin, 40mg / L ampicillin and 25mg / L line Soak in the mixed solution of bacterium ketone for 12h. Put 6 pieces of sterilized fresh rabbit feces directly on the soil after soaking, and after culturing at 30°C for 48 hours, pick the fruiting bodies on the rabbit feces and put them on VY / 2 medium [yeast powder 0.5% (W / V), Ca...

Embodiment 2

[0034] (1) Raw materials: Lemont dry rice stalks, paddy field soil, from Fuzhou, Fujian (teaching farm of Fujian Agriculture and Forestry University);

[0035] (2) Preparation of rice straw powder: 150 g of Lemont dry rice straw was taken, crushed and passed through a 40-mesh sieve to obtain Lemont rice straw powder.

[0036](3) Isolation of myxobacteria: Take 10g of paddy field soil, spread it evenly in a petri dish after natural air drying, and use 40mg / L gentamycin, 10mg / L kanamycin, 40mg / L ampicillin and 25mg / L line Soak in the mixed solution of bacterium ketone for 12h. Put 6 pieces of sterilized fresh rabbit feces directly on the soil after soaking, and after culturing at 30°C for 48 hours, pick the fruiting bodies on the rabbit feces and put them on VY / 2 medium [yeast powder 0.5% (W / V), CaCl2 • 2H2O 0.1%, VB12 0.5μg / mL, Agar 2%, pH 7.2] streaked on the plate and cultured at 30°C for 48h.

[0037] (4) Preparation of culture medium for myxobacteria: Inoculate the myxoba...

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Abstract

The invention provides a method for suppressing the growth of paddy field weeds by virtue of rice straw powder and myxobacteria. The method comprises the following steps: crushing dry rice straws, and sieving and applying rice straw powder into field blocks; inductively separating myxobacteria from soil in a paddy field by adopting a rabbit manure induction method, purifying the myxobacteria, performing enlarging cultivation, mixing the myxobacteria and sterilized crushed rabbit manure, and applying the mixture into the field blocks applied with the rice straw powder to suppress the growth of the paddy field weeds by virtue of interaction of the rice straw powder and the myxobacteria. According to the method, a novel paddy field weeding method which is easy to operate, low in cost and high in ecological safety is established by the technologies, and a reference for the utilization and the development of green and safe weeding of the paddy field can be provided.

Description

technical field [0001] The invention relates to a method for inhibiting the growth of weeds in paddy fields by adding sticky bacteria to rice straw powder, and belongs to the field of biotechnology. Background technique [0002] The harm of weeds in farmland to crop yield and quality is a worldwide problem. Rice is one of the most important crops in my country. In 2014, the sown area of ​​rice in my country was 30.31 million hectares. At the same time, the types of weeds in rice fields in my country are also diverse, including broad-leaved weeds (81 species), Cyperaceae (28 species), Gramineae (15 species) and algae weeds (3 species), while These weeds harm about 15 million hectares of paddy fields in China every year, accounting for about 50% of the total rice planting area. The existence of weeds in paddy fields can reduce rice production by 5% to 15%, and in severe cases it can reach 15% to 30%, or even lose rice. receive. The annual loss of rice due to weed damage in th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G13/00C12N1/20
CPCA01G13/00A01G22/22C12N1/20
Inventor 方长旬林文雄李颖哲李程勋
Owner FUJIAN AGRI & FORESTRY UNIV
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