Method for suppressing growth of paddy field weeds by virtue of rice straw powder and myxobacteria
A technology of myxobacteria and rice stalks, applied in the biological field, can solve the problems of serious food safety hazards, aggravation, biodiversity destruction, etc., and achieve the effect of significant weed suppression, abundant sources, and simple preparation methods
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Embodiment 1
[0026] The specific steps of the method for suppressing weeds in paddy fields by using rice straw powder to add sticky bacteria of the present invention are as follows:
[0027] (1) Raw materials: rice PI312777 dry rice stalks, paddy field soil, from Fuzhou, Fujian (teaching farm of Fujian Agriculture and Forestry University);
[0028] (2) Preparation of rice straw powder: 120 g of PI312777 dry rice straw was taken, crushed and passed through a 40-mesh sieve to obtain PI312777 rice straw powder.
[0029] (3) Isolation of myxobacteria: Take 10g of paddy field soil, spread it evenly in a petri dish after natural air drying, and use 40mg / L gentamycin, 10mg / L kanamycin, 40mg / L ampicillin and 25mg / L line Soak in the mixed solution of bacterium ketone for 12h. Put 6 pieces of sterilized fresh rabbit feces directly on the soil after soaking, and after culturing at 30°C for 48 hours, pick the fruiting bodies on the rabbit feces and put them on VY / 2 medium [yeast powder 0.5% (W / V), Ca...
Embodiment 2
[0034] (1) Raw materials: Lemont dry rice stalks, paddy field soil, from Fuzhou, Fujian (teaching farm of Fujian Agriculture and Forestry University);
[0035] (2) Preparation of rice straw powder: 150 g of Lemont dry rice straw was taken, crushed and passed through a 40-mesh sieve to obtain Lemont rice straw powder.
[0036](3) Isolation of myxobacteria: Take 10g of paddy field soil, spread it evenly in a petri dish after natural air drying, and use 40mg / L gentamycin, 10mg / L kanamycin, 40mg / L ampicillin and 25mg / L line Soak in the mixed solution of bacterium ketone for 12h. Put 6 pieces of sterilized fresh rabbit feces directly on the soil after soaking, and after culturing at 30°C for 48 hours, pick the fruiting bodies on the rabbit feces and put them on VY / 2 medium [yeast powder 0.5% (W / V), CaCl2 · 2H2O 0.1%, VB12 0.5μg / mL, Agar 2%, pH 7.2] streaked on the plate, cultured at 30°C for 48h.
[0037] (4) Preparation of culture medium for myxobacteria: Inoculate the myxobacte...
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