Reverse transcription loop-mediated isothermal amplification test kit of hog cholera virus and application thereof

Abstract

The invention discloses a reverse transcription loop-mediated isothermal amplification test kit of a hog cholera virus and an application thereof. The test kit comprises an RT-LAMP primer, a 2*reaction buffer solution, an EM, a fluorescence visual detection reagent, ultrapure water and a hog cholera virus RNA template. The RT-LAMP primer comprises outer primers F3 and B3, inner primers FIP and BIP and a loop primer LF. The specific detection, the sensibility detection and the fluorescence visual detection prove that by adopting the RT-LAMP detecting method, the hog cholera virus can be specifically detected, the reaction can be real-time monitored, the copy number of the hog cholera virus can be quantitatively determined, the detecting result can be rapidly and accurately obtained, so that the simple, rapid and reliable detection of the hog cholera virus are facilitated.

Description

technical field [0001] The invention relates to the technical field of microorganism detection, in particular to a reverse transcription loop-mediated isothermal amplification kit for rapid, visual and real-time quantitative detection of swine fever virus and its application. Background technique [0002] Swine fever (Hog cholera), also known as Hog Cholera (HC), is commonly known as Intestinal Fever in my country. In order to distinguish it from African swine fever, Europe calls it classical swine fever (Classical swine fever, CSF). The pathogen is Hog cholera virus (HCV or Classical swine fever virus, CSFV), which is a severe contact infectious disease in pigs. Swine fever has been included in the list of Class A diseases by the World Organization for Animal Health (OIE). The disease was first discovered in Ohio in the United States in 1833, and it exists in most swine-raising countries in the world, causing huge economic losses to the swine industry. Although the worl...

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Problems solved by technology

The conventional method is the isolation and identification of the virus, and the result is accurate and reliable, but the conventional identification method has the disadvantages of complex operation, complex equipment and long time, which is not conducive to the rapid diagnosis of swine fever

The method of molecular biology is to detect the specific gene of swine fever virus by reverse transcription polymerase chain reaction (RT-PCR). Although the RT-PCR method is faster and more accurate than the conventional method, it needs to design specific primers and expensive Advanced instruments and equipment are expensive, and agarose gel electrophoresis is required to determine the results, which is likely to cause laboratory pollution and lead to false positive results, and is not suitable for grassroots and on-site testing

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