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Preparation method of photosynthetic bacterium capable of improving yield and/or quality of aquatic animal

A technology for photosynthetic bacteria and aquatic animals, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems such as the disclosure of the technical scheme of the preparation method of Exothorhodospiriosa, and achieve Effects of shortening culture time, promoting growth, and reducing production cost

Inactive Publication Date: 2015-05-27
胡艳晖
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And up to the present, also do not see relevant exothioni rubella preparation method technical scheme open

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0034] A method for preparing photosynthetic bacteria that improves the yield and / or quality of aquatic animals, adopting the species of Rhodospira exothhianospira in the photosynthetic bacteria is cultivated according to the following steps:

[0035] 2. Double-layer flat plate seed activation culture: Streak the strain of Exothorhodospiribacter sasuraui on the solid photosynthetic bacteria medium in the lower layer, then pour a layer of agar medium on it, and culture it under light at 30°C for 5 days, and wait for the streaking The colony turns red and grows a lawn, which can be the activated strain;

[0036] , Seed culture: Inoculate the activated strain into the seed liquid medium, the temperature is 30°C, the light intensity is: 3000lux, light anaerobic culture for 7 days, and the OD660 of the detected seeds is 1.8, which is the seed culture medium;

[0037] , Fermentation culture: Inoculate the seed culture liquid and the fermentation medium with an inoculum ratio o...

example 2

[0044] A method for preparing photosynthetic bacteria that improves the yield and / or quality of aquatic animals, adopting the species of Rhodospira exothhianospira in the photosynthetic bacteria is cultivated according to the following steps:

[0045] 2. Double-layer flat plate seed activation culture: Streak the strain of Exothorhodospiribacter sasuraui on the solid photosynthetic bacteria medium in the lower layer, then pour a layer of agar medium on it, and culture it under light at 30°C for 5 days, and wait for the streaking The colony turns red and grows a lawn, which can be the activated strain;

[0046] , Seed culture: Inoculate the activated strains into the seed liquid culture medium, the temperature is 30°C, the light intensity is: 3000lux, light anaerobic culture for 9 days, and the OD660 of the detected seeds is 2.1, which is the seed culture liquid;

[0047] , Fermentation culture: Inoculate the seed culture liquid and the fermentation medium at an inoculum ...

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Abstract

The invention provides a preparation method of a photosynthetic bacterium capable of improving the yield and / or the quality of aquatic animals, and particularly provides a preparation method of a photosynthetic bacterium preparation good enough to control, minimize or eliminate hydrogen sulfide in water. The preparation method comprises the steps that ectothiorhodospira shaposhnikovii in a purple sulfur photosynthetic bacterium is subjected to seed activating culture, seed culture, and anaerobic fermentation pot illumination culture, and the high-concentration photosynthetic bacterium preparation is obtained. The photosynthetic bacterium preparation can efficiently degrade hydrogen sulfide in the water in aquatic and marine environments, so that hydrogen sulfide in the water is minimized or eliminated, and the yield and / or the quality of the aquatic and marine animals are / is improved.

Description

technical field [0001] The invention belongs to the technical scope of functional microbial preparations, and in particular relates to a preparation method of photosynthetic bacteria for improving the yield and / or quality of aquatic animals. Background technique [0002] Many aquatic and marine environments, such as fish farms, ponds, ponds, lakes, estuaries, and oceans contain one or more aerobic zones and one or more anaerobic zones in the water column. In the aerobic zone near the surface of the water column, air and wind introduce oxygen, while aerobic bacteria produce phosphate, carbon dioxide, and ammonia, among others. In the anaerobic zone near the bottom of the water column, anaerobic microorganisms tend to produce hydrogen sulfide, ammonia, and methane, among others. Accumulations of hydrogen sulfide in anaerobic zones and their underlying sediments are toxic and undesirable because they stress aquatic and marine fauna. In some cases, such as in commercial fish a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/10
CPCC02F3/34C02F2101/101C12N1/205C12R2001/01
Inventor 胡艳晖
Owner 胡艳晖
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