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A kind of extraction process of total triterpenes of fern

An extraction process and a technology for total triterpenes, which are applied in the field of extraction technology of total triterpenes of fern, can solve problems such as complicated steps, no process of removing impurities from starch and sugar, troubles in the purification of total triterpenes, etc., and achieve low production cost, Simple to use effects

Active Publication Date: 2017-06-30
HUZHOU PLATEAU BIOLOGICAL RESOURCE IND INNOVATION CENT OF NORTHWEST INST OF PLATEAU BIOLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current extraction process of total triterpenes is cumbersome and does not involve the removal of starch and sugar
Due to the low content of total triterpenes and high content of starch and sugar in Bracken, a large amount of starch and sugar will be extracted during the extraction process, which will cause great trouble to the purification of total triterpenes in the later stage.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 A kind of extraction process of the total triterpenoids of Bracken, comprising the following steps:

[0020] (1) 0.5 kg of junima root is crushed and passed through a 10-mesh sieve to obtain junima root powder.

[0021] (2) Extract the Juma root powder with ethanol solution with a mass concentration of 50% at a temperature of 60°C, extract 3 times, each time for 0.5 h, and combine to obtain an extract, which is filtered to obtain a filtrate, which is in The extract was obtained by concentrating under reduced pressure at a temperature of 50°C.

[0022] Wherein: the mass volume ratio of the fern root powder and the ethanol solution is 1g:10mL.

[0023] (3) Add water to the extract to settle twice, after stirring, let stand at room temperature for 1 h, then filter to obtain sediment.

[0024] (4) Add absolute ethanol twice its mass to the sediment to dissolve to obtain a solution, add 50% of its volume of activated carbon to decolorize and remove impurities ...

Embodiment 2

[0027] Embodiment 2 A kind of extraction process of the total triterpenoids of Bracken, comprising the following steps:

[0028] (1) After 1kg of junima root is crushed and passed through a 40-mesh sieve, junima root powder is obtained.

[0029] (2) Extract the Juma root powder with ethanol solution with a mass concentration of 60% at a temperature of 70°C, extract 4 times, each time for 1 hour, and combine to obtain an extract, which is filtered to obtain a filtrate, which is in The extract was obtained by concentrating under reduced pressure at a temperature of 70°C.

[0030] Wherein: the mass volume ratio of fern root powder and ethanol solution is 1g:20mL.

[0031] (3) Add water to the extract and settle for 3 times, after stirring, let stand at room temperature for 2 hours, then filter to obtain sediment.

[0032] (4) Add absolute ethanol 5 times its mass to the sediment for dissolution to obtain a solution, add 40% of its volume of activated carbon to decolorize and re...

Embodiment 3

[0035] Embodiment 3 A kind of extraction process of the total triterpenoids of Bracken, comprising the following steps:

[0036] (1) After 10kg of junima root is crushed and passed through a 60-mesh sieve, junima root powder is obtained.

[0037] (2) Extract the Juma root powder with ethanol solution with a mass concentration of 95% at a temperature of 80°C, extract three times, each time for 2.5 hours, and combine to obtain an extract, which is filtered to obtain a filtrate, which is in The extract was obtained by concentrating under reduced pressure at a temperature of 60°C.

[0038] Wherein: the mass volume ratio of the fern root powder and the ethanol solution is 1g:30mL.

[0039] (3) Add water to the extract to settle twice, after stirring, let stand at room temperature for 3 hours, then filter to obtain sediment.

[0040] (4) Add anhydrous ethanol 3 times its mass to the sediment to dissolve to obtain a solution, add 70% of its volume of activated carbon to decolorize ...

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PUM

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Abstract

The present invention relates to a process for extracting total triterpenoids of Junima, which comprises the following steps: (1) Junima root is pulverized and sieved to obtain Junima root powder; (2) Junua root powder is extracted with ethanol solution to obtain an extract , the extract is filtered and concentrated under reduced pressure to obtain an extract; (3) add water to the extract to settle, leave it at room temperature after stirring, and then filter to obtain a sediment; (4) add absolute ethanol to the sediment to dissolve, To obtain a solution, add activated carbon to the solution to decolorize and remove impurities, recover ethanol, dry to constant weight under reduced pressure, and obtain a crude extract; (5) add ether to extract after dissolving the crude extract, stand at room temperature for stratification, repeat 3 Combined after ~5 times to obtain the extract; (6) add n-butanol to the extract for extraction, leave to stand at room temperature for stratification, take the n-butanol solution, repeat the extraction for 4 to 7 times, and combine to obtain the n-butanol solution, the n-butanol solution The butanol solution was dried under reduced pressure to constant weight to obtain the total triterpene extract of Bracken. The invention is simple, efficient and low in cost.

Description

technical field [0001] The invention relates to a process for extracting total triterpenes, in particular to a process for extracting total triterpenes of Bracken. Background technique [0002] The scientific name of Potentilla velvet is Potentilla velvet, a perennial herb belonging to the Rosaceae Potentilla genus. "Potentilla velvet" generally refers to the enlarged root tubers of Potentilla velvet, one of the more commonly used varieties of Tibetan medicine in my country. Root tubers have the effects of promoting body fluid and relieving cough, invigorating the spleen and stomach, astringent and hemostasis, etc. The triterpenoids contained in the root tubers are mainly triterpene acids and triterpene glycosides, which have pharmacological activities such as anti-inflammatory, anti-tumor, antibacterial, and immune regulation . At present, it has been applied in the field of medicine. However, the current extraction process of total triterpenes has cumbersome steps and do...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/73A61P35/00A61K125/00
Inventor 赵晓辉王晓虹韩发
Owner HUZHOU PLATEAU BIOLOGICAL RESOURCE IND INNOVATION CENT OF NORTHWEST INST OF PLATEAU BIOLOGY CHINESE ACADEMY OF SCI
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