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Manufacture of gold plated copper thermode and application of thermode on temperature-controllable H2O2 sensor

A thermal electrode and electrode technology, applied in the field of analysis and detection, can solve problems such as cytotoxicity, and achieve the effects of simple operation, rapid detection, and widening temperature range

Inactive Publication Date: 2015-05-13
FUZHOU UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

But for the vast majority of organisms, H 2 o 2 If the content is too high, it will be toxic to cells

Method used

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  • Manufacture of gold plated copper thermode and application of thermode on temperature-controllable H2O2 sensor
  • Manufacture of gold plated copper thermode and application of thermode on temperature-controllable H2O2 sensor
  • Manufacture of gold plated copper thermode and application of thermode on temperature-controllable H2O2 sensor

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Embodiment Construction

[0018] The present invention will be further described below in conjunction with embodiment. Two (2-hydroxyethyl) amino (trimethylol) methane (Tris), N-(2-hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid) sodium salt (HEPES) used in the present invention Purchased from Sangon Bioengineering (Shanghai) Co., Ltd. Hemin (hemin), tris(2-carboxyethyl)phosphine hydrochloride (TCEP), and 6-mercapto-1-hexanol (MCH) were purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd. Chlorauric acid, sodium nitrate, hydrochloric acid, potassium chloride, ethanol, 30% H 2 o 2 , Nickel sulfate, triethanolamine, sodium chloride, sodium citrate and other reagents were purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd. All reagents were not further purified. The experimental water was ultrapure water purified by the Colton system (resistivity>18 MΩ cm ?1 ). Copper wire was purchased from (3UEW, FUJI TECH, Japan) company, the diameter of copper wire is 600 μm; KW-718 epoxy res...

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Abstract

The invention discloses a manufacture method of a gold plated copper thermode and application of the thermode on a temperature-controllable H2O2 sensor, comprising manufacture of the gold plated copper thermode, formation of a G-quadruplex-hemin complex and a high-sensitivity detection method for hydrogen peroxide by an electrochemical sensor based on the thermode and the G-quadruplex-hemin complex. Based on the high-temperature resistance feature of G-quadruplex-hemin DNA (Deoxyribonucleic Acid) enzyme, electrochemical catalysis response of the hydrogen peroxide on the sensor increases gradually with temperature rising (0-50 degrees centigrade). The electrochemical hydrogen peroxide sensor based on the thermode and G-quadruplex-hemin DNA enzyme provided by the invention can be used in a wide temperature range, has high sensitivity and can realize rapid detection on hydrogen peroxide.

Description

technical field [0001] The invention belongs to the field of analysis and detection, and in particular relates to a method for making a gold-plated copper thermal electrode and a temperature-controllable H 2 o 2 application on the sensor. Background technique [0002] Hydrogen peroxide (H 2 o 2 ) is not only an excellent oxidizing agent but also an important chemical product. It can be used as an oxidizing agent and a disinfectant, and has a wide range of applications in daily life, food processing, and medicine. If hydrogen peroxide enters the human body through food, it may cause cancer of the digestive tract, and it can also accelerate the aging of the human body, which is closely related to the occurrence of various diseases. At the same time in vivo, H 2 o 2 With its intermediate O ? free radical is an important product of many reactions in organisms, H 2 o 2 It is also an intermediate metabolite in many enzymatic reactions, which is closely related to the metab...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 吴韶华陈亮汤优孙建军王芳芳
Owner FUZHOU UNIV
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