Lactobacillus plantarum UCN-11 strain, and composition and application thereof
A technology of Lactobacillus plantarum and composition, applied in the fields of microorganisms, food preservation and animal feed, can solve the problems of blood circulation disorder, pig suffocation and death, etc., and achieve the effects of promoting healthy growth, removing pollution and improving economic benefits.
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preparation Embodiment 1
[0052] Preparation Example 1 Fermentation Culture Preparation
[0053] Take Lactobacillus plantarum UCN-11 strain, inoculate the slope with 20 grams of glucose, 5 grams of corn steep liquor dry powder, 0.5 grams of magnesium sulfate, 0.5 grams of dipotassium hydrogen phosphate, 5 grams of sodium acetate, 2 grams of ammonium citrate, 1000 ml of water, and adjust the pH to 6. 2-6.4, use a pressure cooker to sterilize at 121°C for 15 minutes on a conventional slant, and cultivate the activated strain at 28-30°C for 24 hours.
[0054] The activated strain was inoculated with 20 grams of glucose, 5 grams of corn steep liquor dry powder, 0.5 grams of magnesium sulfate, 0.5 grams of dipotassium hydrogen phosphate, 5 grams of sodium acetate, 2 grams of ammonium citrate, and 1000 ml of water. Cultivate at 30°C for 16-18 hours in the conventional liquid medium prepared after sterilizing for 15 minutes at °C as seed liquid.
[0055] Inoculate 20 grams of glucose, 5 grams of corn steep...
preparation Embodiment 2
[0057] Preparation Example 2 Fermentation Culture Composition Preparation
[0058] Take Lactobacillus plantarum UCN-11 strain, inoculate the slope with 20 grams of glucose, 5 grams of corn steep liquor dry powder, 0.5 grams of magnesium sulfate, 0.5 grams of dipotassium hydrogen phosphate, 5 grams of sodium acetate, 2 grams of ammonium citrate, 1000 ml of water, and adjust the pH to 6. 2-6.4, use a pressure cooker to sterilize at 121°C for 15 minutes on a conventional slant, and cultivate the activated strain at 28-30°C for 24 hours.
[0059] The activated strain was inoculated with 20 grams of glucose, 5 grams of corn steep liquor dry powder, 0.5 grams of magnesium sulfate, 0.5 grams of dipotassium hydrogen phosphate, 5 grams of sodium acetate, 2 grams of ammonium citrate, and 1000 ml of water. Cultivate at 30°C for 16-18 hours in the conventional liquid medium prepared after sterilizing for 15 minutes at °C as seed liquid.
[0060] Inoculate 20 grams of glucose at 1-5%, 5...
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