Tissue culture propagation method for reducing tissue culture browning rate of traxacum koksaghyz

Abstract

The invention belongs to the field of plant tissue culture of plant cell engineering techniques, and in particular relates to a tissue culture propagation method for reducing the tissue culture browning rate of traxacum koksaghyz. The tissue culture propagation method comprises the following steps: inoculating, namely, selecting mature seeds of a traxacum koksaghyz strain; performing induced differentiation on calluses; inducing callus formation; proliferating adventitious buds; performing rooting culture; separating multiple shoots into single plants, and transferring to a rooting culture medium for rooting culture; performing acclimatization and transplantation, thereby obtaining regenerated plants of traxacum koksaghyz. By adopting the tissue culture propagation method, a method for effectively reducing the tissue culture browning rate of traxacum koksaghyz and rapidly propagating traxacum koksaghyz is provided, the tissue culture browning rate of traxacum koksaghyz is greatly reduced through technical measures such as adjusting components of culture mediums used in different stages and adding an antioxidant, the propagation coefficient of traxacum koksaghyz is improved, the browning rate can be reduced to be less than 35% when the culture mediums provided by the invention is adopted, and the browning rate can be further reduced to be less than 10% when Na2S2O3 is added.

Description

technical field [0001] The invention belongs to the field of plant tissue culture of plant cell engineering technology, and specifically relates to a tissue culture propagation method for reducing the browning rate of Russian rubber grass tissue culture. Background technique [0002] Rubber grass (Taraxacum kok-saghyz Rodin) is a perennial herbaceous plant of the genus Dandelion in the Compositae family. The average natural rubber content in its roots is 22.4%, and its quality is similar to that produced by Hevea brasiliensis. It also contains 25-40% chrysanthemum. Sugar is recognized as one of the excellent natural rubber plants with development potential, and it is also an ideal model plant for studying the regulation of rubber synthesis and storage molecular processes. [0003] During the propagation process of rubber grass, self-fertilization is sterile, so its seeds are the result of hybridization between individuals and different strains, so it is difficult to use seed...

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Problems solved by technology

[0003] During the propagation process of rubber grass, self-fertilization is sterile, so its seeds are the result of hybridization between individuals and different strains, so it is difficult to use seeds to preserve excellent individuals and genetic lines for further breeding; at the same time, because the wild habitat of rubber grass has been severely damaged, At present, its resources are very scarce, which limits its experimental research, so it is necessary to develop a tissue culture method, and at the same time provide technical support for the establishment of the genetic transformation system of rubber grass and its breeding

At present, my country's research includes: "Research on Tissue Culture of Rubber Grass" published in "Tropical Agricultural Engineering" by Lin Bohuang, Institute of Rubber, Chinese Academy of Tropical Agricultural Sciences, and "Establishment of High Frequency Regeneration System of Rubber Grass" published by Luo Chenghua, Shihezi University, "Northern Horticulture" etc. According to the analysis of the current research results, most of the existing technologies use the wild rubber grass in Xinjiang as the material. The explants of rubber grass leaves are directly sterilized and inoculated onto the medium, which can easily lead to browning, especially in the subtropical and temperate climates and soil environments of mainland my country, the browning rate is as high as 80%. Survival rate is also low

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