Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method and kit for detecting polymorphism of XRCC1 gene

A gene polymorphism and kit technology, applied in the field of molecular biology, can solve problems such as toxic side effects, treatment and prognosis effects, and low efficiency, and achieve the effect of saving test samples and low test costs

Inactive Publication Date: 2015-05-06
SHANGHAI PASSION BIOTECHNOLOGY CO LTD
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the field of malignant tumor treatment, due to the lack of selectivity of chemotherapy for tumor tissue and normal tissue, and the same chemotherapy regimen shows different sensitivities among different individuals, it leads to low efficiency and toxic side effects in most tumor chemotherapy treatments. Serious and important reasons, studies have shown that small changes in the nucleotide sequence of DNA molecules can have an impact on the treatment and prognosis of a variety of solid tumors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and kit for detecting polymorphism of XRCC1 gene
  • Method and kit for detecting polymorphism of XRCC1 gene
  • Method and kit for detecting polymorphism of XRCC1 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Extraction of genomic DNA samples to be tested

[0026] In this embodiment, human blood is extracted as genomic DNA to be detected, and Tiangen Blood Genomic DNA Extraction Kit is used for extraction.

[0027] 2. Design and synthesis of upstream and downstream primers

[0028] Synthesize the upstream primer shown in SEQ ID NO:1 and the downstream primer shown in SEQ ID NO:2:

[0029] SEQ ID NO: 1: 5'-ACCTGGGGATGTCTTGTTGA-3'

[0030] SEQ ID NO: 2: 5'-GAGTGAAAAGGGTCTTGGGC-3'.

[0031] 3. Determine the annealing temperature by pre-experiment

[0032] The annealing temperature was determined by using the ERCC1 primers designed and synthesized above as a temperature gradient. attached figure 1 It is the gel electrophoresis image of the pre-experiment. It can be seen from the image that when the annealing temperature is 70°C, the gel strip is single, the specificity is the strongest, and the strip is very bright. When the annealing temperature is other temperature, the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of molecular biology and particularly relates to a method and kit for detecting the polymorphism of an XRCC1 gene. The method comprises the following steps: (1) by adopting to-be-detected genomic DNA as a template, designing and synthesizing an upstream primer having the sequence as shown in SEQ ID NO: 1 and a downstream primer having the sequence as shown in SEQ ID NO: 2 and carrying out specific PCR amplification; (2) carrying out agarose gel electrophoresis on PCR amplification products and then carrying out gel recovery; and (3) sequencing the gel recovery products and comparing the sequencing results to determine the polymorphism of the gene. The primer pair used in the invention has stronger specificity, after the target gene is subjected to PCR amplification by virtue of the primer pair, the agarose gel electrophoresis is performed to obtain bands with very high teleonomy so that the success rate and reliability of the detection method are improved and the detection of polymorphic sites is of important significance for guiding individual treatment of cancer.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a method and a kit for detecting XRCC1 gene polymorphism. Background technique [0002] XRCC1 is an important DNA repair gene, and its encoded XRCC1 protein will participate in the process of base excision repair. XRCC1 single nucleotide polymorphisms are associated with the efficacy of chemotherapy drugs such as cisplatin or carboplatin. The main mechanism of action of platinum-based drugs is to cause DNA replication disorders, resulting in apoptosis of cancer cells. Enhanced DNA repair ability is the main cause of platinum drug resistance. XRCC1 is an important component in the base repair system. The single nucleotide polymorphism in its conserved region will affect the function of XRCC1 protein, improve DNA repair function, and cause platinum resistance. The relationship between XRCC1 and XRCC1 gene polymorphisms and platinum There is a close relationship among t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2535/101C12Q2565/125
Inventor 孙子奎高文学丁方美王锋仇伟
Owner SHANGHAI PASSION BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com