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Microalgae breeding device and microalgae breeding method

A breeding method and microalgae technology, applied in the field of microalgae cultivation, can solve the problems of affecting production, net production decline, light energy loss, etc., and achieve the effects of increasing overall production, reducing ventilation energy consumption, and avoiding shear damage

Inactive Publication Date: 2015-05-06
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although the high-density cultivation in the prior art can improve the light uniformity of algae cells and the light utilization efficiency of high-intensity illumination by increasing the mixing intensity, the high-density cultivation technology in the prior art also has the following problems: (1) If a culture device with a narrow optical path is used, the light cannot be fully utilized after passing through the culture device, resulting in light energy loss; but if the light does not penetrate the culture device, it is necessary to increase the culture density of algae cells , but this method will make it difficult to match the cell density with the light intensity and the mixing intensity: if the mixing is insufficient, the algal cells will receive uneven light energy; if the mixing is too strong, the shear force generated will damage the algal cells and increase The density of algae cells will also consume a lot of respiration of algae cells, resulting in a decrease in net yield; (2) If a culture device with a wide optical path is used, the movement path of algae cells will be longer, which is not conducive to the rapid reception of light energy by algae cells for photosynthesis At the same time, the wide optical path is often accompanied by high-intensity mixing requirements, which consumes a lot of energy, and the shear force generated by high-intensity mixing will also cause damage to algae cells and affect the increase in production.
[0005] Therefore, although the high-density culture technology in the prior art can improve the utilization efficiency of high-intensity light, there are problems such as high mixing intensity requirements and damage to algae cells; but if the mixing intensity of algae cells is guaranteed to be low, it will not If damage is caused, high-intensity light cannot be fully utilized, which will cause light energy loss and reduce light utilization efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Microalgae cultivation device: using such as figure 2 The shown height is a double-layer cylindrical nested silicone reactor of H, including two breeding layers of an inner layer and an outer layer, wherein the inner layer optical path (i.e. inner layer diameter) is 3cm, and the outer layer optical path (i.e. Thickness) 1cm.

[0105] Inoculation density: Prepare the medium, concentrate the algae and inoculate it into the reactor. The inoculation density gradually decreases from the outer layer to the inner layer of the reactor, respectively: the inoculation density of the outer layer is 60OD, and the inoculation density of the inner layer is 20OD.

[0106] Aeration form: Aeration is carried out with ring-shaped aeration tubes. The inner and outer layers of the reactor are respectively provided with a set of ring-shaped aeration tubes. During the breeding process, carbon dioxide and air are respectively added to the inner and outer layers of the reactor. Aeration with ...

Embodiment 2

[0126] Microalgae cultivation device: using such as image 3 The shown length is L, height is the double-layer plate type nested silicone reactor of H, comprises inner layer and outer layer two breeding layers, and wherein, inner layer optical distance (that is inner layer thickness) 4cm, outer layer optical path The distance (ie thickness) is 2cm.

[0127] Inoculation density: Prepare the medium, concentrate the algae and inoculate it into the reactor. The inoculation density gradually decreases from the outer layer to the inner layer of the reactor, respectively: the inoculation density of the outer layer is 30OD, and the inoculation density of the inner layer is 15OD.

[0128] Aeration form: Aeration is carried out with ring-shaped aeration tubes. The inner and outer layers of the reactor are respectively provided with a set of ring-shaped aeration tubes. During the breeding process, carbon dioxide and air are respectively added to the inner and outer layers of the reactor....

Embodiment 3

[0134] The microalgae culture is carried out in basically the same manner as in Example 2, the only difference being that the light intensity of each group of light sources in Example 3 is 1000 μmol / m 2 / s.

[0135] Experimental results

[0136] The 5-day average output of embodiment 2 is about 7.5g / day, and the 5-day average output of embodiment 3 is about 8.4g / day, and the 5-day average output of comparative example 2 is about 6.0g / day; Embodiment 2 is relatively Ratio 2 output improves 25%, and embodiment 3 improves 40% compared with comparative example 2 output, simultaneously, embodiment 3 improves 12% compared with embodiment 2 output; But the overall energy consumption of embodiment 2, embodiment 3 is comparative example 29.2% of 2. The energy consumption calculation method is the same as the energy consumption calculation method of the above-mentioned first group of experiments, and will not be repeated here.

[0137] The experimental results are consistent with the...

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Abstract

The invention discloses a microalgae breeding device and a microalgae breeding method, relating to the technical field of microalgae breeding. According to the microalgae breeding device, while the light utilization efficiency of high-intensity lighting is improved, high mixing strength is not required, and the damage of shearing force, caused by high-strength mixing, to algal cells is further avoided; the microalgae breeding device comprises a photobioreactor which comprises at least two breeding layers, and an aeration device is arranged in each breeding layer or an aeration device and a stirring device are both arranged in each breeding layer. The microalgae breeding device and the microalgae breeding method are suitable for the microalgae breeding process.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to a microalgae cultivation device and method. Background technique [0002] When microalgae are cultivated, the light utilization rate of microalgae depends on the amount of light absorbed by the algal cells. High-intensity light is a prerequisite for high yield, but there is a light saturation point in the absorption and utilization of light by algae cells. When the light intensity is below the light saturation point, the light utilization efficiency of algae cells will decrease, and too much light energy will be lost in the form of heat and fluorescence, and even cause damage to cells with a lower light saturation point. Therefore, in the cultivation process, in order to ensure that each algal cell can be evenly irradiated by light, it is necessary to aerate or stir and mix, so that the algae cells can undergo a "light-dark cycle" in the cultivation system, that i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/04C12M1/02C12N1/20C12R1/89
CPCC12M21/02C12M23/44C12M27/02C12M29/06C12N1/12
Inventor 冯倩石蕾杨建强耿金峰陈彦平马欣欣
Owner ENN SCI & TECH DEV
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