Bottle gourd virus resistance-related gene segment or gene markers and application of gene segments or gene markers

A technology of gene fragments and gene markers, applied in the field of plant biotechnology science, can solve problems affecting the growth and development of gourd plants, economic losses, etc., and achieve the effects of rapid identification, shortening the breeding period, and improving breeding efficiency

Inactive Publication Date: 2015-04-29
CHANGSHU ZHILIN FRUITS & VEGETABLES PROFESSIONAL COOP
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Problems solved by technology

The virus disease affects the normal growth and development pr...

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  • Bottle gourd virus resistance-related gene segment or gene markers and application of gene segments or gene markers
  • Bottle gourd virus resistance-related gene segment or gene markers and application of gene segments or gene markers
  • Bottle gourd virus resistance-related gene segment or gene markers and application of gene segments or gene markers

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Embodiment Construction

[0017] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. The method can quickly identify the gourd anti-virus disease gene. The specific implementation process is as follows:

[0018] 1. DNA extraction: Take an appropriate amount of young gourd leaves, dry them with silica gel, grind them into powder, and extract the total genomic DNA with the CTAB method. The extracted DNA was detected by an ultraviolet spectrophotometer, and samples with an OD260 / OD280 value of 1.8-2.0 were selected and stored at -20°C for future use.

[0019] 2. Amplification and cloning of the homologous fragment of the disease resistance gene: according to the amino acid sequence GG(L / I / V / M / S)GKTT and G(L / N) of the conserved region of the known NBS-LRR type disease resistance gene ) PL(A / T / S)(L / V), designed a pair of degenerate primers: NBS15'-GKTT GGIGGIWSIGGIAARACIACG-3'...

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Abstract

The invention mainly relates to bottle gourd virus resistance-related gene segments or gene markers and application thereof. A disease-resistant gene homologous sequence method is mainly adopted for rapidly separating three bottle gourd virus resistance-related gene segments or gene markers, sequencing is carried out, and lengths of nucleotides of the gene segment or gene markers are 510bp, 510bp and 504bp respectively. The bottle gourd virus resistance-related gene segments or gene markers have the advantages that a bottle gourd virus resistance-related gene mining period is effectively shortened, and rapid identification of a virus disease gene is facilitated; corresponding coseparation functional markers (SNP, SCAR and the like) are developed by virtue of the identified virus disease gene, the identified virus disease gene also can be used for virus disease resistant gene molecular marker-assisted selection rapidly with high accuracy; by combining with other disease-resistant gene molecular markers, a multi-resistance breeding material can be created, breeding cycle can be shortened, and breeding efficiency can be improved; and a foundation is laid for explaining a bottle gourd virus resistance-related gene molecular mechanism.

Description

technical field [0001] The invention utilizes the homologous sequence method of disease-resistant genes to clone gourd anti-viral disease gene fragments or convert them into molecular markers, etc., and mainly relates to gourd conventional hybrid breeding, molecular marker-assisted breeding, positioning and molecular markers of anti-viral disease genes. Mapping, cloning of anti-viral disease genes, and analysis of the regulation mechanism of disease-resistant genes belong to the field of plant biotechnology science. Background technique [0002] Gourd is one of the important vegetable crops in the middle and lower reaches of the Yangtze River. Viral diseases are the most important diseases in gourd production. The disease mainly occurs at the initial stage of colonization, causing the plants to be short, the leaves shrunk, the plants cannot grow normally, and in severe cases, the leaves turn yellow or die later. The disease occurs at the adult stage, and the leaves appear ...

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Application Information

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IPC IPC(8): C12N15/29C12N15/11C12Q1/68
Inventor 钱孝英钱春桃
Owner CHANGSHU ZHILIN FRUITS & VEGETABLES PROFESSIONAL COOP
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