Quick identification of MLO powdery mildew resistant gene for citrus
A powdery mildew resistance gene and citrus technology, applied in genetic engineering, plant genetic improvement, biochemical equipment and methods, etc., can solve the problems of long time and difficult cloning, and achieve the goal of shortening the breeding period, shortening time, and low efficiency Effect
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[0031] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. This method can quickly identify the citrus powdery mildew gene. The specific implementation process is as follows:
[0032] 1) Collection and identification of citrus MLO-type genes
[0033] In order to obtain all the MLO gene family members of citrus, we first constructed an HMM model using the MLO gene sequences of Arabidopsis, tomato, pea, pepper, rose, pepper, and lotus root MLO gene sequences for resistance to powdery mildew. Search for MLO-type genes; secondly, use the published MLO gene sequences in different crops as target sequences (from DFCI database: TC171015, TC267529, DFCI: TC327983, TC289653, TC312087, TC132500, TC133436, TC317623, TC317025, TC315947, TC325903, TC315944,TC315912,TC322759,TC322059,TC330654,TC282713,TC293173,TC281861,TC283253,TC283383,TC285032,TC290021,TC302...
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