Towel gourd virus resistance-related gene segment or gene marker and application

A technology of gene markers and gene fragments, applied in the field of plant biotechnology science, can solve problems affecting the growth and development of loofah plants, economic losses, etc., and achieve the effects of rapid identification, shortening the breeding period, and improving breeding efficiency

Inactive Publication Date: 2015-04-29
CHANGSHU CITY DONGBANG DONGDUN VEGETABLE PROFESSION COOP
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AI Technical Summary

Problems solved by technology

Viral diseases have seriously affected the normal growth and development process of loofah plants, bringing great economic losses to production

Method used

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  • Towel gourd virus resistance-related gene segment or gene marker and application
  • Towel gourd virus resistance-related gene segment or gene marker and application
  • Towel gourd virus resistance-related gene segment or gene marker and application

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Experimental program
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Embodiment Construction

[0017] The identification of disease-resistant genes plays an important role in the theoretical research of crop disease-resistant genetics and the selection of disease-resistant varieties. The method can quickly identify the loofah anti-virus disease gene. The specific implementation process is as follows:

[0018] 1. DNA extraction: Take an appropriate amount of young leaves of loofah, dry them with silica gel, grind them into powder, and extract the total genomic DNA by CTAB method. The extracted DNA was detected by an ultraviolet spectrophotometer, and samples with an OD260 / OD280 value of 1.8-2.0 were selected and stored at -20°C for future use.

[0019] 2. Amplification and cloning of the homologous fragment of the disease resistance gene: according to the amino acid sequence GG(L / I / V / M / S)GKTT and G(L / N) of the conserved region of the known NBS-LRR type disease resistance gene ) PL(A / T / S)(L / V), designed a pair of degenerate primers: NBS15'-GKTT GGIGGIWSIGGIAARACIACG-3' ...

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Abstract

The invention mainly relates to a towel gourd virus resistance-related gene segment or gene marker and an application. A virus resistance-related gene homologous sequencing method is used for quickly separating 3 towel gourd virus resistance-related gene segments or gene markers, and by means of sequencing, the nucleotide lengths of these gene segments or gene markers are respectively 504bp, 498bp and 498 bp. By adopting the invention, the mining cycle of the towel gourd virus resistance-related gene is effectively shortened to help the rapid identification of virogenes; the identified virogenes are used for developing corresponding co-separation functional markers (SNP, SCAR and the like), the identified virogenes can also be applied to the auxiliary selection of the molecular markers of the virogenes, and the accuracy is high; in combination with other anti-disease gene molecular markers, a multi-resistance breeding material can be cultured to shorten the breeding period and improve the breeding efficiency; and a foundation is laid for illustrating the molecular mechanism of the towel gourd virus resistance-related genes.

Description

technical field [0001] The invention utilizes the homologous sequence method of the disease-resistant gene to clone loofah anti-viral disease gene fragments or convert them into molecular markers, etc., and mainly relates to conventional hybrid breeding of loofah, molecular marker-assisted breeding, positioning and molecular mapping of anti-viral disease genes , the cloning of anti-viral disease genes and the analysis of the regulatory mechanism of disease-resistant genes belong to the field of plant biotechnology science. Background technique [0002] Viral diseases are one of the most important diseases in loofah production. Young leaves are susceptible to light green and dark green mottled or faded green small ring spots. Old leaves are infected with yellow ring spots or yellow and green mosaic leaves, and the veins shrink, causing the leaves to be twisted or deformed. The leaves with severe disease become hard and brittle, and the notch on the leaf edge deepens, and de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 钱孝英钱春桃
Owner CHANGSHU CITY DONGBANG DONGDUN VEGETABLE PROFESSION COOP
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