Preparation method of herceptin modified and quantum dot/ferroferric oxide loaded magnetic fluorescent silica nanoparticles

A technology of ferroferric oxide and silicon dioxide, applied in chemical instruments and methods, nanotechnology for materials and surface science, nanotechnology, etc., can solve problems such as complex synthesis processes, and achieve high optical strength and low toxicity , The effect of stabilizing light fastness

Inactive Publication Date: 2015-04-29
TIANJIN POLYTECHNIC UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, this technique requires complex synthesis and specific modification by antibodies, and the captured cells often need to be isolated for detection

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0013] Dissolve 50 μL of quantum dots in chloroform at a concentration of 50 μM, then pour 5 ml of 0.55 mM CTAB aqueous solution into the above solution, and then vigorously stir the resulting solution for 30 min to form an oil-in-water microemulsion. The aqueous solution was then heated to 60°C to remove chloroform by evaporation, and then 3 ml of ethyl orthosilicate and 3 ml of ethyl acetate were added to the above reaction solution and stirred for 3 hours. The silicon dioxide nanoparticles loaded with quantum dots are repeatedly washed with ethanol to remove unreacted reactants, and the nanospheres coated with silicon dioxide quantum dots can be obtained. Example 2:

Embodiment 2

[0014] For surface modification of silicon dioxide, 10 mg of silicon dioxide nanoparticles and 4 ml of triethoxysilane were dissolved in 10 ml of absolute ethanol, and refluxed at 70° C. for 3 hours. For surface modification of ferric oxide, 1.3g BMPA and 15mg Fe 3 o 4 Nanoparticles were dissolved in 10ml of n-hexane, and the reaction was continued for 72 hours under nitrogen protection flow. To conjugate Fe3O4 nanoparticles to QD-loaded silica nanoparticles, 1000N Fe 3 o 4 Dissolve the silica nanoparticles loaded with quantum dots in ethanol solution with 20 μL triethylamine, heat the reactant to 75°C and reflux for 24 hours under the protection of nitrogen, and centrifuge the obtained reactant with ethanol to remove excess Fe 3 o 4 , the nanoparticles of quantum dots coated with silicon dioxide conjugated with ferric oxide can be obtained.

Embodiment 3

[0016] Dissolve Herceptin in PBS solution and dilute to 1mg / ml, shake the antibody and nanoparticles at room temperature overnight and mix, after centrifugation and further washing with PBS solution, the polyhedral oligomeric fold modified by Herceptin can be obtained Semioxosilane-conjugated quantum dots-silica / ferric oxide composite nanoparticles.

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Abstract

The invention relates to a preparation method of herceptin modified and quantum dot / ferroferric oxide loaded magnetic fluorescent silica nanoparticles. The prepared composite nanoparticles can be used for separating and detecting circulating tumor cells. The preparation method of the herceptin modified and quantum dot / ferroferric oxide loaded magnetic fluorescent silica nanoparticles comprises steps as follows: a POSS (polyhedral oligomeric silsesquiaxana) conjugated quantum dot is prepared firstly, the POSS conjugated quantum dot is coated with silica spheres, Br-IO is conjugated to the outer layer of QD-SiNP, finally, herceptin is conjugated to IO-QD-SiNP, and the herceptin modified and quantum dot / ferroferric oxide loaded magnetic fluorescent silica nanoparticles are obtained. The composite nanoparticles prepared with the method have fluorescence, magnetism and target functions and are expected to be applied to in-vivo and in-vitro bioimaging.

Description

technical field [0001] The invention relates to a method for preparing Herceptin-modified polyhedral oligomeric silsesquioxane conjugated quantum dot-silicon dioxide / ferric iron tetroxide nanoparticles. Background technique [0002] Cancer is a major cause of death in the world and is difficult to cure due to its metastatic nature, which means that cancer cells can detach from the initial tumor cells to blood vessels and then to different tissues in the body. Cancer cells detached from the original tumor tissue and then circulated in the surrounding blood vessels are known as circulating tumor cells. Isolation and detection of these cells will help us in the early diagnosis of cancer, which is crucial for patient diagnosis and monitoring of treatment effects. [0003] Several methods have been used to isolate and enumerate circulating tumor cells, for example, immunomagnetic cell sorting and fluorescence-activated cell sorting are the two most commonly used commercial metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C09K11/02B82Y30/00B82Y40/00H01F1/11C12N5/09
Inventor 潘杰卞雨霞万冬卢涛
Owner TIANJIN POLYTECHNIC UNIV
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