Special strain medium for liquefaction of Pleurotus osmanthus and corresponding culture method
A strain culture medium and strain culture technology, applied in the directions of application, fungi, botanical equipment and methods, etc., can solve the problem that liquid fermentation strains cannot be applied on a large scale, the production accident of fruiting bag pollution, and the increase of fruiting bags. Pollution risks and other problems, to achieve the effect of eliminating secondary pollution, solving the cultivation of seed sources, and reducing the amount of inoculation
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Embodiment 1
[0038] Embodiment 1, a kind of culture medium special for the liquefaction of pear mushroom, it is made up of the composition of following parts by weight:
[0039]
[0040] The preparation method of above-mentioned culture medium is to carry out following steps successively:
[0041] In a stainless steel container, cornstarch, fine bran (wheat bran), and wheat cellulose are mixed uniformly under dry conditions to obtain ingredient I;
[0042] Add glucose, yeast extract, beef peptone, potassium dihydrogen phosphate, and magnesium sulfate into water, and fully stir (dissolve glucose, yeast extract, beef peptone, potassium dihydrogen phosphate, and magnesium sulfate in water) to obtain ingredient II;
[0043] After ingredients Ⅰ and Ⅱ are fully mixed, the culture medium for liquefaction of mushrooms is obtained.
[0044] Remarks: The special strain culture medium for the liquefaction of Zhenzhen mushrooms is prepared and used now.
Embodiment 2
[0045] Embodiment 2, utilize the liquefaction bacterial classification culture method of Pleurotus chinensis to carry out by the substratum of embodiment 1 gained, the Pleurotus chinensis parent kind is carried out following steps successively:
[0046] 1), strain production:
[0047] 200g of the special strain culture medium for liquefaction of the now-prepared Pleurotus osmanthus is immediately distributed in the special culture bottle of 200ml, and the microporous membrane ventilated cover is covered (selecting ZP14-200 ventilated culture bottle of Shanghai Songtuo Industrial Co., Ltd.), at 121 ℃, 0.11Mpa under high temperature and high pressure sterilization for 90 minutes; get the culture medium after sterilization;
[0048] Insert 5 grams of Pleurotus officinalis parent species (solid parent species) into the above-mentioned sterilized medium under aseptic conditions, and cultivate in the dark at 24° C. for 20 days to obtain a solid bacterial species for liquefaction;
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