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Special strain medium for liquefaction of Pleurotus osmanthus and corresponding culture method

A strain culture medium and strain culture technology, applied in the directions of application, fungi, botanical equipment and methods, etc., can solve the problem that liquid fermentation strains cannot be applied on a large scale, the production accident of fruiting bag pollution, and the increase of fruiting bags. Pollution risks and other problems, to achieve the effect of eliminating secondary pollution, solving the cultivation of seed sources, and reducing the amount of inoculation

Active Publication Date: 2017-11-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Remarks: The liquid fermentation strain process theoretically has a high purity of the strain, and the bacterial contamination rate of large-scale fermentation production is between 1% and 5% (in a 3-stage fermenter), and the bacterial contamination (mainly bacterial) is often In the later stage of fermentation, it is difficult to find out by conventional on-line inspection, which leads to production accidents caused by the inoculation of liquid fermentation strains and the contamination of large quantities of mushroom bags, which is a painful lesson.
In addition, because the cultivation of liquid strains requires nutrients such as high-concentration organic nitrogen and sugar sources, these ingredients remain in the bacterial liquid and are inserted into the fruiting bag. Since bacteria are inevitably brought in during operation, the residual nutrients It becomes a breeding ground for miscellaneous bacteria, which increases the risk of contamination after inoculation of the fruiting bag
This is the main problem that liquid fermentation strains cannot be applied to the production of edible fungi on a large scale

Method used

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  • Special strain medium for liquefaction of Pleurotus osmanthus and corresponding culture method
  • Special strain medium for liquefaction of Pleurotus osmanthus and corresponding culture method
  • Special strain medium for liquefaction of Pleurotus osmanthus and corresponding culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1, a kind of culture medium special for the liquefaction of pear mushroom, it is made up of the composition of following parts by weight:

[0039]

[0040] The preparation method of above-mentioned culture medium is to carry out following steps successively:

[0041] In a stainless steel container, cornstarch, fine bran (wheat bran), and wheat cellulose are mixed uniformly under dry conditions to obtain ingredient I;

[0042] Add glucose, yeast extract, beef peptone, potassium dihydrogen phosphate, and magnesium sulfate into water, and fully stir (dissolve glucose, yeast extract, beef peptone, potassium dihydrogen phosphate, and magnesium sulfate in water) to obtain ingredient II;

[0043] After ingredients Ⅰ and Ⅱ are fully mixed, the culture medium for liquefaction of mushrooms is obtained.

[0044] Remarks: The special strain culture medium for the liquefaction of Zhenzhen mushrooms is prepared and used now.

Embodiment 2

[0045] Embodiment 2, utilize the liquefaction bacterial classification culture method of Pleurotus chinensis to carry out by the substratum of embodiment 1 gained, the Pleurotus chinensis parent kind is carried out following steps successively:

[0046] 1), strain production:

[0047] 200g of the special strain culture medium for liquefaction of the now-prepared Pleurotus osmanthus is immediately distributed in the special culture bottle of 200ml, and the microporous membrane ventilated cover is covered (selecting ZP14-200 ventilated culture bottle of Shanghai Songtuo Industrial Co., Ltd.), at 121 ℃, 0.11Mpa under high temperature and high pressure sterilization for 90 minutes; get the culture medium after sterilization;

[0048] Insert 5 grams of Pleurotus officinalis parent species (solid parent species) into the above-mentioned sterilized medium under aseptic conditions, and cultivate in the dark at 24° C. for 20 days to obtain a solid bacterial species for liquefaction;

...

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Abstract

The invention discloses a special strain culture medium for pleurotus geesteranus liquefaction. The special strain culture medium is prepared from the following components in parts by weight: 45-50 parts of corn starch, 1-2 parts of glucose, 15-20 parts of wheat cellulose, 20-25 parts of fine bran, 1-1.5 parts of a yeast extract, 1-1.5 parts of beef peptone, 2-3 parts of potassium dihydrogen phosphate, 1-1.5 parts of magnesium sulfate and 100 parts of water. The invention further simultaneously discloses a strain culture method for carrying out pleurotus geesteranus liquefaction by employing the culture medium. The culture method sequentially comprises the following steps: carrying out high-temperature and high-pressure sterilization on a special strain culture medium for pleurotus geesteranus liquefaction; inoculating the sterilized strain culture medium into a pleurotus geesteranus mother strain in the sterilized culture medium under a sterile condition, and carrying out darkness culture at 23-25 DEG C for 19-21 days; and diluting the obtained special solid strain for liquefaction, so as to obtain the liquefied pleurotus geesteranus strain.

Description

technical field [0001] The invention relates to a formula and a cultivation method of special bacteria for liquefaction of Pleurotus osmanthus. Background technique [0002] Edible fungus is one of the advantageous industries with the most modern agricultural development characteristics in China. This is not only because China is the world's largest producer and consumer of edible fungi, with a wide range of edible fungus production, but more importantly, the edible fungus industry is a good carrier and prominent position in the industrialization of biotechnology and the sustainable development of agriculture. At present, my country's edible fungus industry system has many factors that are not suitable for the global market economy, the most important of which are the intensification of industrial operations, low degree of specialization, backward production factors, imperfect production technology, and lack of key technology support. The most representative one is the inten...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00A01G1/04
CPCC12N1/14
Inventor 陈再鸣何伯伟陈青余维良郑明海
Owner ZHEJIANG UNIV
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