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Nano composite contrast agent for photoacoustic imaging and preparation method thereof

A nano-composite and photoacoustic imaging technology, which is applied in echo/ultrasonic imaging agents, preparations for in vivo tests, pharmaceutical formulations, etc., can solve problems such as poor biocompatibility, weak photoacoustic signals, and large toxic and side effects , to achieve good photoacoustic imaging effect, strong photoacoustic signal and high loading rate

Inactive Publication Date: 2015-04-29
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of this, the present invention provides a nanocomposite contrast agent for photoacoustic imaging, which combines the advantages of reduced graphene oxide and the organic dye indocyanine green ICG to solve the biophase problem of the photoacoustic imaging contrast agent in the prior art. Poor capacitance, poor stability, weak photoacoustic signal, and serious side effects

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  • Nano composite contrast agent for photoacoustic imaging and preparation method thereof
  • Nano composite contrast agent for photoacoustic imaging and preparation method thereof
  • Nano composite contrast agent for photoacoustic imaging and preparation method thereof

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Embodiment 1

[0047] A method for preparing a nanocomposite contrast agent for photoacoustic imaging, comprising the following steps:

[0048] (1) Graphene oxide modified with polyethylene glycol:

[0049] a. Take 20mg of graphene oxide, add 10mL of water, and ultrasonically disperse in a water bath for 1h (ultrasonic power is 600W), to obtain a colloidal solution of nanometer graphene oxide (NGO); 1.2g of sodium hydroxide and 1.0g of chloroacetic acid Add in the colloidal solution of NGO, sonicate 30min (ultrasonic power is 500W), obtain reaction mixture, the hydroxyl group and epoxy group on NGO surface are converted into-COONa at this moment; Said reaction mixture is diluted to 1mg / mL, And transferred to a 30kDa dialysis bag, dialyzed with deionized water until the pH of the dialyzed fluid is neutral to obtain an aqueous solution of carboxylated graphene oxide (NGO-COOH);

[0050] b. Take 20 mL of the NGO-COOH aqueous solution obtained in step (a) (adjust the concentration to 1 mg / mL), ...

Embodiment 2

[0060] A method for preparing a nanocomposite contrast agent for photoacoustic imaging, comprising the following steps:

[0061] (1) Graphene oxide modified with polyethylene glycol:

[0062] a. Take 20mg of graphene oxide, add 10mL of water, and ultrasonically disperse in a water bath for 2h (ultrasonic power is 600W), to obtain a colloidal solution of nano-graphene oxide (NGO); 1.2g of sodium hydroxide and 1.0g of chloroacetic acid Added in the colloidal solution of NGO, ultrasonic crushing 60min (ultrasonic power is 500W), obtains reaction mixture, the hydroxyl group and epoxy group on NGO surface are converted into-COONa at this moment; The reaction mixture is diluted to 1gm / mL, And transferred to the dialysis bag of 40kDa, with deionized water dialyzed to the pH value of the dialyzed external fluid is neutral, obtains the aqueous solution of carboxylated graphene oxide (NGO-COOH);

[0063] b. Take 20 mL of the NGO-COOH aqueous solution obtained in step (a) (adjust the co...

Embodiment 3

[0070] A method for preparing a nanocomposite contrast agent for photoacoustic imaging, comprising the following steps:

[0071] (1) Graphene oxide modified by polyglycol:

[0072]a. Take 20mg of graphene oxide, add 10mL of water, and ultrasonically disperse in a water bath for 3h (ultrasonic power is 600W), to obtain a colloidal solution of nanometer graphene oxide (NGO); 1.2g of sodium hydroxide and 1.0g of chloroacetic acid Added in the colloidal solution of NGO, ultrasonic crushing 45min (ultrasonic power is 500W), obtains the reaction mixture, the hydroxyl group and the epoxy group on the NGO surface are converted into-COONa at this moment; The reaction mixture is diluted to 1gm / mL, And transferred to the dialysis bag of 40kDa, with deionized water dialyzed to the pH value of the dialyzed external fluid is neutral, obtains the aqueous solution of carboxylated graphene oxide (NGO-COOH);

[0073] b. Take 20 mL of the NGO-COOH aqueous solution obtained in step (a) (adjust t...

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Abstract

The invention provides a nano composite contrast agent for photoacoustic imaging. The nano composite contrast agent for photoacoustic imaging provided by the invention comprises indocyanine green (ICG) and polyethylene glycol modified reduced graphene oxide; and the indocyanine green is bonded to the surface of the polyethylene glycol modified reduced graphene oxide through phi-phi conjugate action. The invention further provides a preparation method of the nano composite contrast agent. The preparation method provided by the invention comprises the following steps of modifying aminated polyethylene glycol on the surface of graphene oxide, using an in-situ reduction method to obtain polyethylene glycol modified reduced graphene oxide, and loading ICG by using the reduced graphene oxide so as to obtain the nano composite contrast agent for photoacoustic imaging. The nano composite contrast agent provided by the invention not only has good biocompatibility, long retention time in a body and high ICG loading rate but also has stronger optoacoustic signals; and in addition, because the ICG and the reduced graphene oxide can enhance absorption in a near infrared region with each other, more nontoxic ICG can be loaded by using little reduced graphene oxide.

Description

technical field [0001] The invention belongs to the technical field of photoacoustic imaging contrast agents, and in particular relates to a nanocomposite contrast agent for photoacoustic imaging and a preparation method thereof. Background technique [0002] Photoacoustic imaging is a non-destructive medical imaging method developed in recent years. It mainly performs reconstruction imaging by measuring the ultrasonic waves generated after the absorption of pulsed laser by biological tissue. It combines the high contrast of pure optical imaging with the high penetration of pure ultrasound imaging, and has great application prospects in high-resolution imaging of tumor tissue and function. However, due to the strong scattering effect of the laser light, the intensity of the incident laser light and the photoacoustic signal-to-noise ratio will decrease exponentially with the increase of the depth of the biological tissue. In order to overcome these difficulties, a contrast ag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/22
Inventor 陈敬钦宋亮刘成波曾光王慧娜闫盟
Owner SHENZHEN INST OF ADVANCED TECH
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