Culturing method of momordica cochinchinensis

Abstract

The invention discloses a culturing method of momordica cochinchinensis. The culturing method comprises the following steps: 1, differentiation culture, namely, inoculating a momordica cochinchinensis explant into an MS differentiation culture medium for 25-35 days until lateral buds of the explant is differentiated, wherein the culturing temperature is 23-26 DEG C, the illumination intensity is 1400-2000lux, and the illumination period is 10-12h / day; 2, strong seedling culture, namely, culturing the momordica cochinchinensis explant in an MS strong seedling culture medium for 35-45 days to obtain a momordica cochinchinensis strong plant, wherein the culturing temperature is 23-26 DEG C, the illumination intensity is 1400-2000lux, and the illumination period is 10-12h / day; and 3, rooting culture, namely, culturing the momordica cochinchinensis strong plant in an MS strong seedling culture medium for 35-45 days to obtain a rooted momordica cochinchinensis plant, wherein the culturing temperature is 23-26 DEG C, the illumination intensity is 1400-2000lux, and the illumination period is 10-12h / day. According to the method disclosed by the invention, a large number of momordica cochinchinensis seedlings suitable for being transplanted can be immediately propagated.

Description

technical field [0001] The invention relates to a method for plant propagation, in particular to a method for cultivating wood turtles. Background technique [0002] Wood turtle (Momordica cochinchinensis (Lour) Spreng) is a dioecious perennial herbaceous vine belonging to the family Cucurbitaceae, which has important ornamental and medicinal values. With saponins, orcitonin, sterols, alkaloids, etc. as the main active ingredients, it has broad development and application prospects in surgical medicine, insecticidal pesticides, etc. Scorpion is mainly distributed in Guangxi, Sichuan, Guangdong, Jiangxi, Hunan, Hubei, Yunnan, Guizhou and other provinces in China, and it is cultivated in most provinces and regions in the south (Chen Xiaojun, Cultivation Technology of Scorpion, Southern Horticulture, 2010, 03: 54 -55.). [0003] Wood softshell turtles are often propagated by seeds and root tubers; however, the number of seeds is limited, the germination and seedling rate is l...

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Problems solved by technology

[0003] Wood softshell turtles are often propagated by seeds and root tubers; however, the number of seeds is limited, the germination and seedling rate is low, and because of dioeciousness, the genetic background of the offspring of seedlings is heterozygous, the population is uneven, and it is difficult to guarantee the flowering and fruiting of the year; root tubers Propagation is usually cut into several small pieces according to the number of bud eyes on the tuber root, each piece has at least one bud eye, buried in the sowing hole, one piece for each hole, generally can bloom and bear fruit in the same year, faster than seed propagation, but the number of reproduction is also limited (Edited by Zhao Shiwei , Landscape Design of Garden Engineering: Encyclopedia of Plant Configuration and Cultivation Application (Volume 1, 2 and 2), China Agricultural Science and Technology Press, 1st Edition, September 2000, p. 1457)

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