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Method for solving flowering asynchronism during crossbreeding of cymbidium

A technology of hybrid breeding with no flowering period, which is applied in the field of plant propagation, can solve the problems of breeding with no flowering period, etc., and achieve the effects of enhanced competitiveness, strong vitality, and high success rate of hybridization

Inactive Publication Date: 2015-04-29
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The pollen with vitality can be used for artificial pollination on the stigma of the female parent, and then the orchid hybrid seedlings can be obtained through the steps of cultivating hybrid fruits, aseptic sowing and transplanting test tube seedlings, so as to effectively solve the problem of flowering period in orchid hybrid breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: crossbreeding and seedling propagation of Doritis pulcherrima (Doritis pulcherrima) and Yunnan Flame Orchid (Renanthera imschootiana)

[0012] (1) Artificial pollination: Yunnan Flame Orchid blooms in early March. Collect pollen when the petals of its single flower are just unfolded. Stick the pollen block on the tip of the toothpick with a toothpick and quickly put the pollen block into a clean and sterile 1ml EP tube, put the pollen block of 1 flower in each tube, put the pollen block in the EP tube and use a sterile glass The bottles were collected and sealed, and finally the glass bottles filled with pollen were placed at 4°C (low temperature preservation layer of the refrigerator) and stored away from light. After the flame orchid pollen was preserved for 3 months, when the female parent orchid orchid bloomed in early June of that year, the pollen of the flame orchid preserved at low temperature was thawed and rehumidified to restore and improve the v...

Embodiment 2

[0017] Embodiment 2: crossbreeding and seedling propagation of small flower Wandailan (Vanda coerulescens) and Yunnan Flame Orchid (Renanthera imschootiana)

[0018] (1) Artificial pollination: Yunnan Flame Orchid blooms in early March. Collect pollen when the petals of its single flower are just unfolded. Stick the pollen block on the toothpick tip with a toothpick and quickly put the pollen block into a clean and sterile 1ml EP tube, put the pollen block of 1 flower in each tube, put the pollen block in the EP tube and use a sterile glass bottle Pack them together and seal them well, and finally store the glass bottles filled with pollen at 4°C and avoid light. After the flame orchid pollen has been stored for 6 months, when the female parent floret Vanda orchid blooms in early September of that year, the low-temperature preserved flame orchid pollen will be thawed and rehumidified to restore and improve the vitality of the pollen. Thaw the flame orchid pollen stored at low...

Embodiment 3

[0023] Embodiment 3: the crossbreeding and seedling propagation of Caixin Jianlan (Cymbidium ensifolium) and Yunnan Flame Orchid (Renanthera imschootiana)

[0024] (1) Artificial pollination: Yunnan Flame Orchid blooms in early March, collect pollen when its single flower petals just unfold, use a sterilized toothpick to gently pick off the medicinal cap of the flower at 8-9 in the morning, and then use the toothpick to remove the pollen. The pollen block sticks to the tip of the toothpick and quickly put the pollen block into a clean and sterile 1ml EP tube, put the pollen block of 1 flower in each tube, put the pollen block in the EP tube and pack it in a sterile glass bottle Put it up and seal it well, and finally place the glass bottle filled with pollen at 6°C and keep it away from light. After the flame orchid pollen has been preserved for 9 months, when the female parent Caixin Jianlan blooms in early December of that year, the low-temperature preserved flame orchid polle...

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Abstract

The invention discloses a method for solving flowering asynchronism during crossbreeding of cymbidium. The method includes the following steps: picking out an anther cap of cymbidium through a sterilized picking tool when the petal of a cymbidium simple flower unfolds; picking the cymbidium pollinium to a clean and sterile container through the sterilized picking tool and sealing the pollinium in the sterile container; storing the container in a dark place at the temperature of 4-6 DEG C; unfreezing the stored pollinium at the room temperature for 20-30 min when the female parent orchid blooms; adding sterile water and performing rewetting for 20-30 min to obtain the vital pollen. According to the invention, the cymbidium pollinium stored as per the method provided by the invention can be stored for 3-12 months without going mouldy; after unfreezing and rewetting, and measured by the 2,3,5-triphenyltetrazolium chloride staining method, the vitality of the pollen stored according to the method is high, the success rate of hand pollination is high, and the hybridization success rate is high.

Description

Technical field: [0001] The invention belongs to the field of plant propagation, and in particular relates to a method for solving the failure of flowering period in orchid cross breeding. Background technique: [0002] Orchid is a famous and precious flower that is popular all over the world. It is one of the most widely cultivated and sold flower species in the world. It has developed particularly rapidly in recent years, with an annual growth rate of more than 10%. One hundred million U.S. dollars. The Netherlands, Thailand, Singapore, the United States, South Korea, and Japan are countries with relatively developed orchid industries, and Thailand is the largest exporter of orchids. At present, most of the orchids produced are moth orchids, dendrobium orchids and Cattleya and other orchids, while the national orchids with traditional Chinese characteristics are not large in scale. my country is currently one of the largest producers of orchids, and our province is the m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 吴坤林曾宋君张建霞段俊
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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