Peak-shape front extension suppressor, high-performance liquid chromatography analysis device and method using same

Abstract

The invention discloses a peak-forward suppressor for a high-performance liquid chromatography analysis device and an analysis method, comprising a ferrule and a core arranged inside the ferrule, the core is a hollow structure and A cavity is provided, and a sieve plate is provided at the end inside the core, and the sieve plate communicates with the cavity. Also disclosed is a high-performance liquid chromatography analysis device and method using the peak shape forward suppression, so that when the sample solvent carrying the sample flows through the cavity, the sample solvent will be diluted by the mobile phase. Advantages of the present invention: it can effectively suppress the peak shape leading phenomenon caused by "mismatch between sample solvent and mobile phase", solve the trouble caused by "sample solvent", improve peak shape, increase column efficiency, and improve separation Spend. It greatly reduces the interference caused by the sample solvent during analysis, preparation and method development, and greatly saves manpower and material resources.

Description

technical field [0001] The invention relates to the technical field of chromatographic analysis, in particular to high-performance liquid chromatography analysis and preparation, and in particular to a peak-forward suppressor, a high-performance liquid chromatography analysis device and a method using the same. Background technique [0002] High performance liquid chromatography is currently the most important means and method in the process of production supervision and quality control in biological, pharmaceutical, medical, food, chemical and other industries. The world includes biopharmaceuticals, medical diagnosis, food safety and chemical quality inspection and other fields. More than 80% of the tens of thousands of compounds in the country are analyzed by high performance liquid chromatography, which is widely involved in various fields of national economy and people's livelihood. With the development of the times and the improvement of people's living standards, peopl...

AI Technical Summary

Problems solved by technology

In the process of developing the method, many samples have poor solubility in the mobile phase. However, due to the limitation of some objective conditions, it is difficult to dissolve the sample with the mobile phase. In many cases, it is necessary to use a solvent different from the mobile phase to dissolve the sample. Therefore, It causes the phenomenon that the peak shape is often prone to prolongation during liquid chromatography analysis and preparation, which seriously affects the accuracy of peak area integration, resulting in a sharp drop in column efficiency and a significant reduction in resolution when liquid chromatography is used for analysis and preparation. The efficiency becomes worse, the content calculation is very inaccurate, and it will directly affect the separation purity in the preparation liquid phase

[0009] Such a major impact makes some methods have to be changed, and the changing process will increase the cost of the process and pre-treatment, and some even have to give up all the work done before, and start from scratch to find the method, wasting a lot of manpower and material resources resource

[0010] Many of the current high-performance liquid chromatography methods in the national standard and pharmacopoeia are troubled by the large difference between the composition of the "sample solvent" and the mobile phase, which makes many analysts who need to use these methods to test their products greatly confused. Headache, because the method is authoritative, it cannot be easily changed, and the "sample solvent" in these methods does not match the mobile phase, so people have to constantly try new columns without changing the original method, hoping to have a new column. This magic column will solve their problem, but it will not be easy, and even if the problem is finally solved, this process will consume a lot of time, effort, expensive solvents, etc. Columns, resulting in a huge waste of resources

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