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Silk screen printing type multilocus enzyme electrode and preparation method and application of silk screen printing type multilocus enzyme electrode

A technology of screen printing electrodes and screen printing, which is applied to electrochemical variables of materials, material analysis by electromagnetic means, measuring devices, etc., can solve the problems of incomplete detection materials, easy occurrence of false positives and false negatives, etc. The method is simple, the detection sensitivity and measurement range are improved, and the cost is low.

Inactive Publication Date: 2015-04-22
YANGTZE DELTA REGION INST OF TSINGHUA UNIV ZHEJIANG
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Problems solved by technology

The disadvantage of the single-point enzyme electrode is mainly that the detection substance is incomplete, or it is prone to false positive or false negative

Method used

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  • Silk screen printing type multilocus enzyme electrode and preparation method and application of silk screen printing type multilocus enzyme electrode
  • Silk screen printing type multilocus enzyme electrode and preparation method and application of silk screen printing type multilocus enzyme electrode
  • Silk screen printing type multilocus enzyme electrode and preparation method and application of silk screen printing type multilocus enzyme electrode

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Embodiment Construction

[0030] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments. Unless otherwise specified, all are conventional methods; the experimental materials used, unless otherwise specified, were purchased from conventional biochemical reagent manufacturers.

[0031] A screen printing multi-site enzyme electrode, comprising a base electrode, enzymes are immobilized at multiple sites on the surface of the base electrode; the base electrode is a screen printing electrode; the enzyme is an oxidase, including horseradish Oxidase (HRP) and Laccase.

[0032] The preparation method of the screen-printed multi-site enzyme electrode comprises the steps of: printing the carbon electrode layer with carbon paste, and after the carbon electrode layer is printed, put the carbon electrode layer in an oven at 90°C and keep it warm for 30 minutes; then print Insulation layer, after the insulation layer is printed, put it in an oven at 9...

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Abstract

The invention discloses a silk screen printing type multilocus enzyme electrode and a preparation method and application of the silk screen printing type multilocus enzyme electrode. The silk screen printing type multilocus enzyme electrode comprises a base electrode, wherein enzymes are fixed to multiple positions on the surface of the base electrode, the base electrode is a silk screen printing electrode, and the enzymes are oxidizing enzymes. By means of the enzyme electrode, osmium polymers are fixed to the four-position electrode through glutaraldehyde and BSA crosslinking by analyzing phenolic substances through the combination of various types of oxidizing enzymes after the osmium polymers are mixed with HRP and laccase, H2O2 is added for assistance, phenolic compounds which commonly and strongly react to the two enzymes are detected, and the good analysis capacity and the good anti-interference capacity are provided for the phenolic compounds. The preparation method is simple, low in cost and simple in operation process, and the electron transferring speed between electrons and the enzymes is high; the more beneficial online long-term real-time detection environment is provided; the detection sensitivity of a biosensor is improved, the detection range of the biosensor is widened, and the detection result can be quicker and more accurate.

Description

technical field [0001] The invention belongs to the technical field of novel biosensors, in particular to a screen-printed multi-site enzyme electrode and its preparation method and application. Background technique [0002] In the field of biosensors, enzyme electrodes play an important role. Enzyme electrode refers to immobilizing the active substance enzyme on the surface of the electrode to detect the current-type or potential-type catalytic reaction signal. It has the molecular recognition and catalytic function of the enzyme, and has the characteristics of fast response and easy operation of the electrochemical electrode, and can quickly measure the test solution. The concentration of a given compound in . [0003] On the basis of enzyme electrode research, the method of electron mediator modification is introduced. Different electron mediators, such as small molecule dyes, ferrocene compounds, redox polymers, etc., can change the electrode potential and promote the p...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N27/26
Inventor 黄灿灿蔡强
Owner YANGTZE DELTA REGION INST OF TSINGHUA UNIV ZHEJIANG
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