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Virus immunized inducer and application thereof

An inducer and virus technology, applied in the field of plant virus immune induction technology, can solve the problems of yield and quality decline, vulnerability to virus damage, insufficient active immunity, etc.

Active Publication Date: 2015-04-22
武汉勤劳农夫生态农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although virus-free strawberry seedlings do not contain viruses, they are still vulnerable to viruses during planting in nature. The active immunity of seedling plants to viruses is insufficient, and virus infection will lead to a decline in yield and quality

Method used

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  • Virus immunized inducer and application thereof
  • Virus immunized inducer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0014] Taking the Virus-free Seedlings of Farland Strawberry as an Example

[0015] 1. Weigh raw materials (KG) according to the following weight ratio: 3.2KG of astragalus polysaccharide, 2.6KG of chlorogenic acid, 2.6KG of salvia ketone, 1.6KG of motherwort, and mix the above raw materials evenly.

[0016] 2. Add 8.5% of the above-mentioned virus immune inducer in the preparation process of rooting medium for tissue culture of strawberry virus-free seedlings.

[0017] 3. Strawberry stem tip or root tip detoxification method, cultivate 2000 subculture seedlings to be rooted under the same environmental conditions, and use PCR method to detect virus and confirm that there is no virus.

[0018] 4. Prepare the rooting medium added with the immune inducer, cultivate and obtain 1000 rooting original seedlings as the experimental group A. It was observed that the rooting of virus-free strawberry seedlings was not affected when the immune inducer was added, and the rooting original...

experiment example 2

[0024] Taking virus-free seedlings of red strawberry as an example

[0025] 1. Weigh the raw materials (kg) according to the following weight ratio: 3.2 kg of astragalus polysaccharide, 2.6 kg of chlorogenic acid, 2.6 kg of salvia ketone, 1.6 kg of motherwort, and mix the above raw materials evenly.

[0026] 2. Add 8.5% of the above-mentioned virus immune inducer in the preparation process of rooting medium for tissue culture of strawberry virus-free seedlings.

[0027] 3. Strawberry stem tip or root tip detoxification method, cultivate 2000 subculture seedlings to be rooted under the same environmental conditions, and use PCR method to detect virus and confirm that there is no virus.

[0028] 4. Strawberry stem tip or root tip detoxification method, cultivate 2000 subcultured seedlings to be rooted under the same environmental conditions, and use PCR method for virus detection to confirm that there is no virus.

[0029] 5. Prepare a rooting medium added with an immune induce...

Embodiment 3

[0036] 1. Take the raw materials (kg) according to the following weight ratio: 1.8kg of astragalus polysaccharide, 1.6kg of konjac glucomannan, 3.5kg of sterilized humic acid, 1.2kg of salvia ketones, 1.6kg of catarrhine, motherwort Alkali 1.6kg, mix above-mentioned raw material evenly.

[0037] 2. Adding 12.5% ​​of the above-mentioned virus immune inducer in the preparation process of rooting medium for strawberry virus-free seedling tissue culture.

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Abstract

The invention discloses a virus immunized inducer and application thereof, and belongs to the technical field of plant virus immunized induction. The inducer is prepared by the following steps: uniformly mixing the following components in percentage by weight: 32 percent of astragalus polysaccharide, 26 percent of chlorogenic acid, 26 percent of total tanshinones and 16 percent of leonurine; and adding 8.5 percent of the mixture in a root medium for strawberry virus-free seedling tissue culture. The immunized inducer has the advantages of effectively improving the resistance of strawberry virus-free seedlings on viruses, has excellent immunity on strawberry mottle virus, crinkle virus and vein banding virus in the natural planting process, and has minor immunity on strawberry mild yellow edge virus.

Description

technical field [0001] The invention relates to a plant virus immunity inducer and its application in strawberry detoxification immunity induction, belonging to plant virus immunity induction technology. Background technique [0002] Strawberries are easily infected with a variety of viruses in the process of planting in nature, which makes the offspring of natural breeding congenitally defective, mainly manifested in the degradation of resistance, yield and quality. There are usually four kinds of viruses infected, namely strawberry mottle virus and light yellow edge virus. , Vein Virus and Shrunken Virus. In recent years, detoxification of strawberries has become a mature technology. Although virus-free strawberry seedlings do not contain viruses, they are still vulnerable to viruses during planting in nature. The active immunity of seedling plants to viruses is insufficient, and virus infection will lead to a decline in yield and quality. Therefore, it is necessary to i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/22A01N47/44A01P21/00A01N37/38A01N43/16
Inventor 刘开红
Owner 武汉勤劳农夫生态农业有限公司
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