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Fusarium oxysporum BM201, compound pectinase produced by fusarium oxysporum BM201 and preparation method and application of compound pectinase

A technology of Fusarium oxysporum and compound pectinase, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problem of large water consumption of acid-base capsules, easy contamination of orange juice cells, and reduced product safety Sexuality and other issues, to achieve the effect of low cost, avoid low production efficiency and improve production efficiency

Inactive Publication Date: 2015-04-08
SHAOYANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The process of decapsulation in navel orange processing currently mainly adopts the traditional acid-base treatment process of canned citrus, which requires manual peeling and splitting, and the production efficiency is low; the water consumption of acid-base capsules is large, and the environment is seriously polluted; the orange juice cells after decapsulation It is easy to be polluted, which reduces the safety of the product; the existing decapsulation process is not suitable for processing the peeled navel orange pellets with a thick capsule and a tightly combined structure between the capsule and the fruit

Method used

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  • Fusarium oxysporum BM201, compound pectinase produced by fusarium oxysporum BM201 and preparation method and application of compound pectinase
  • Fusarium oxysporum BM201, compound pectinase produced by fusarium oxysporum BM201 and preparation method and application of compound pectinase
  • Fusarium oxysporum BM201, compound pectinase produced by fusarium oxysporum BM201 and preparation method and application of compound pectinase

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preparation example Construction

[0078] In the preparation method, the fermentation broth can be prepared according to conventional methods. Preferably, the step of preparing a fermentation broth includes an activation step, a seed cultivation step and a fermentation step.

[0079] Preferably, the activation step is to inoculate the strain on the activation medium, culture it at 28°C-32°C for 2-3 days, and prepare the obtained spores into a spore suspension of 1.5×105-2×106 spores / mL. Activation under these conditions can activate the strains in the strain to the greatest extent and improve its enzyme production activity. The medium used in the activation step is the same as the aforementioned activation medium.

[0080] Preferably, in the seed culture step, inoculate the spore suspension of 3-5% of the bottled amount into the seed medium and cultivate it for 36 hours at 28-32° C. and 120-150 r / min to obtain the seed bacterium liquid. The bottling volume here refers to the volume ratio of the added spore su...

Embodiment 1

[0102] (1) Activation: After the frozen-preserved Fusarium oxysporum (Fusarium oxysporum) BM201 strain was activated on a solid slant surface, it was formulated with sterilized deionized water into a 1.5×10 5 per mL of spore suspension. The activation temperature is 28°C, and the activation time is 2 days.

[0103] Activation medium: dry powdered navel orange pomace, citrus pomace and pomelo peel waste or a mixture of any species 8g / L, (NH 4 ) 2 SO 4 1.5g / L, K 2 HPO 4 1.0g / L, KCl0.5g / L, MgSO 4 0.5g / L, FeSO 4 0.1g / L, EDTA iron sodium salt 0.01g / L, ox bile salt 0.5g / L, agar 1.5%, pH5.0.

[0104] (2) Seed culture: get the spore suspension of 3%% of the bottled amount in the shaker bottle and insert it into the seed medium, at 28°C, 120r / min, cultivate it for 36h as the seed bacterium liquid produced by large-scale fermentation;

[0105] The medium used for seed cultivation: navel orange pomace, citrus pomace and pomelo peel waste or a mixture of any species 15g / L, (NH 4 ...

Embodiment 2

[0111] (1) Activation: After the frozen-preserved Fusarium oxysporum (Fusarium oxysporum) BM201 strain was activated on a solid slant surface, it was mixed with sterilized deionized water to prepare 2 × 10 6 per mL of spore suspension. The activation temperature is 32°C, and the activation time is 3 days.

[0112] Activation medium: dry powdered navel orange pomace, citrus pomace and pomelo peel waste or a mixture of any species 10g / L, (NH 4 ) 2 SO 4 2.0g / L, K 2 HPO 4 1.5g / L, KCl1.0g / L, MgSO 4 0.8g / L, FeSO 4 0.2g / L, EDTA iron sodium salt 0.02g / L, ox bile salt 0.8g / L, agar 2%, pH6.5.

[0113] (2) Seed culture: get 5% spore suspension of the bottled amount in the shake bottle and insert it into the seed culture medium, at 32°C, 150r / min, cultivate it for 36h as the seed bacterium liquid produced by large-scale fermentation;

[0114] The medium used for seed cultivation: navel orange pomace, citrus pomace and pomelo peel waste or a mixture of any species 20g / L, (NH 4 ) ...

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Abstract

The invention provides fusarium oxysporum BM201, compound pectinase produced by the fusarium oxysporum BM201 and a preparation method and application of the compound pectinase. The fusarium oxysporum is fusarium oxysporum BM201; the Latin Name of the fusarium oxysporum is Fusarium oxysporum; the preservation number of the fusarium oxysporum is CGMCC No.9108. The Fusarium oxysporum BM201 provided by the invention is capable of simultaneously producing multiple pectinases such as polygalacturonase, pectate lyase, pectinesterase, cellulase and hemicellulase to form the compound pectinase. By virtue of coordinated proportion and mutual action of various enzymes in the compound pectinase, the removal rate of the peels of the fruits can be increased when the compound pectinase is utilized for removing peels of plants and fruits, and the production efficiency can be improved. The strain can be further used for decomposing waste residues such as fruit peels, so that the environmental pollution is alleviated, and the waste is recycled.

Description

technical field [0001] The invention relates to the field of pectinase-producing bacteria, in particular to Fusarium oxysporum BM201, a compound pectinase produced by it, and a preparation method and application of the compound pectinase. Background technique [0002] Pectin is a linear polymer formed by connecting multiple D-lacturonic acids through α-1,4 glycosidic bonds. Pectin substances widely exist in the primary wall and intercellular space of plants, and constitute the glue in the middle layer of adjacent cells. At the same time, pectin substances can also interweave with non-starch polysaccharides such as cellulose and hemicellulose to form cell walls. [0003] Pectinase is a compound enzyme capable of decomposing pectin-like substances. Since pectin-like substances are widely present in various parts of plant cells, pectinase is used to decompose pectin in plant cells in the prior art. To destroy plant cell structure and plant tissue structure. Pectinase can be ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/88C12N9/42C12N9/26C12N9/18A23L2/02A23L2/84A23L1/212A23L1/015C12R1/77A23L5/20A23L19/00
CPCA23L2/04A23L2/84A23N7/01C12N1/14C12N9/18C12N9/2402C12N9/2434C12N9/2437C12N9/88C12Y301/01011C12Y302/01015C12Y402/0201C12N1/145C12R2001/77
Inventor 尹乐斌赵良忠李文李陈李海鸥杨汝希卜宇芳刘倩
Owner SHAOYANG UNIV
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