Preparation method of chlorella pyrenoidosa polyphenol
A technology of chlorella pyrenoidosa and its operation method, which is applied in the field of preparation of chlorella pyrenoidosa polyphenols, and achieves the effects of safe process flow, convenient industrial production, and low production cost
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Embodiment 1
[0019] A preparation method of Chlorella pyrenoidosa polyphenols is characterized in that: the main process flow is: pulverization, mixing, extraction, and filtration, and the specific operation method sequentially follows the following steps:
[0020] (1) Crushing: Collect Chlorella pyrenoidosa, wash, dry, and crush through a 40-mesh sieve;
[0021] (2) Mixing: Weigh Chlorella pyrenoidosa, then add an ethanol solution with a concentration of 10-80%, with a material-to-liquid ratio of 20-100ml / g, stir evenly to prepare a mixed solution;
[0022] (3) Extraction: The above-mentioned mixed liquid is extracted in a microwave field with a microwave power of 500-1500W, and the microwave processing time is 20-100s to prepare an extraction filtrate;
[0023] (4) Filtration: freeze-drying the above-mentioned filtrate to obtain a polyphenol extract of Chlorella pyrenoidosa.
Embodiment 2
[0025] Extract seaweed polyphenols as follows:
[0026] (1) Collect Chlorella pyrenoidosa, wash, dry, and crush through a 40-mesh sieve;
[0027] (2) Weigh out Chlorella pyrenoidosa, then add a 30% ethanol solution with a material-to-liquid ratio of 10ml / g, and stir properly.
[0028] (3) The mixture of step 2 is extracted in a microwave field with a microwave power of 800W, and the microwave processing time is 20s.
[0029] (4) Filter, freeze-dry the filtrate to obtain polyphenol extract.
[0030] The extraction rate of seaweed polyphenols is 2.51%.
Embodiment 3
[0032] Extract seaweed polyphenols as follows:
[0033] (1) Collect Chlorella pyrenoidosa, wash, dry, and crush through a 40-mesh sieve;
[0034] (2) Weigh out Chlorella pyrenoidosa, and then add a 20% ethanol solution with a material-to-liquid ratio of 20ml / g, and stir properly.
[0035] (3) The mixture of step 2 is extracted in a microwave field with a microwave power of 800W, and the microwave processing time is 40s.
[0036] (4) Filter, freeze-dry the filtrate to obtain polyphenol extract.
[0037] The extraction rate of seaweed polyphenols was 3.08%.
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