Monosodium glutamate production technology
A production process and monosodium glutamate technology, applied in the fields of application, food preparation, food science, etc., can solve problems such as extinction of aquatic organisms, and achieve the effects of reducing production costs, high economic benefits, and improving economic benefits
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Embodiment 1
[0051] A kind of monosodium glutamate production technology, comprises the following steps:
[0052] 1) Secondary seed culture: 10 m 3 Seed tank capacity 7 m 3 Culture medium, inoculate FM415 strain slant into primary medium for primary culture, the mass percentage formula of primary medium is: medical oral glucose 2.5%, urea 0.55%, dipotassium hydrogen phosphate 0.12%, corn steep liquor 3.0 %, magnesium sulfate 0.06%, tap water 93.77%, primary seed cultivation conditions: shaker culture temperature 32°C-34°C, pH value 6.8-7.3, shaker frequency 100 times / min, culture cycle 9.5 hours;
[0053] Inoculate the first-grade seeds obtained by cultivating into the second-level medium for secondary cultivation to obtain the second-level seeds. The mass percentage formula of the second-level medium is: taking starch as raw material and adopting double-enzyme method to produce glucose 5.1%, tap water 91.3% %, Potassium Dihydrogen Phosphate 0.2%, Magnesium Sulfate 0.1%, Corn Steep Steep...
Embodiment 2
[0060] A kind of monosodium glutamate production technology, comprises the following steps:
[0061] 1) Secondary seed culture: 10 m 3 Seed tank capacity 7 m 3 Culture medium, inoculate FM415 strain slant into primary medium for primary culture, the mass percentage formula of primary medium is: medical oral glucose 2.5%, urea 0.55%, dipotassium hydrogen phosphate 0.12%, corn steep liquor 3.0 %, magnesium sulfate 0.06%, tap water 93.77%, primary seed cultivation conditions: shaker culture temperature 32-34°C, pH value 6.8-7.3, shaker frequency 100 times / min, culture cycle 9.5 hours;
[0062] Inoculate the first-grade seeds obtained by cultivation into the second-grade medium for secondary cultivation to obtain the second-grade seeds. The mass percentage formula of the second-grade medium is: taking starch as raw material and adopting double-enzyme method to produce glucose 5.1%, tap water 91.3% %, Potassium Dihydrogen Phosphate 0.2%, Magnesium Sulfate 0.12%, Corn Steep Steep ...
Embodiment 3
[0069] A kind of monosodium glutamate production technology, comprises the following steps:
[0070] 1) Secondary seed culture: 10 m 3 Seed tank capacity 7 m 3 Culture medium, inoculate FM415 strain slant into primary medium for primary culture, the mass percentage formula of primary medium is: medical oral glucose 2.5%, urea 0.55%, dipotassium hydrogen phosphate 0.12%, corn steep liquor 3.0 %, magnesium sulfate 0.06%, tap water 93.77%, primary seed cultivation conditions: shaker culture temperature 32-34°C, pH value 6.8-7.3, shaker frequency 100 times / min, culture cycle 10 hours;
[0071] Inoculate the first-grade seeds obtained by cultivation into the second-grade medium for secondary cultivation to obtain the second-grade seeds. The mass percentage formula of the second-grade medium is: taking starch as raw material and adopting double-enzyme method to produce glucose 5.1%, tap water 91.3% %, Potassium Dihydrogen Phosphate 0.2%, Magnesium Sulfate 0.1%, Corn Steep Steep 2....
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