A method for the determination of chlorantraniliprole residues in fruits and vegetables

Abstract

The invention discloses a method for measuring the residual quantity of chlorantraniliprole in fruit and vegetable. The method comprises performing homogeneous extraction on chlorantraniliprole residue in a sample through acetonitrile or an acetonitrile solution containing 1% of acetic acid; performing dispersed purification on extracted solution through ethylenediamine-N-propyl silicane (PSA) and octadecyl silane bonded phase (C18) matrix; then performing gas chromatograph-negative chemical ionization-mass spectrometry (GC-NCI-MS) detection, establishing a calibration standard curve through blank matrix solution not containing pesticide to be measured, and performing quantitation through the external standard method. The method for measuring the residual quantity of the chlorantraniliprole in fruit and vegetable achieves an average recovery rate of 90.7-101.9%, an average relative standard deviation (RSD) of 5.4-9.6% and a detection limit lower than 0.77 mu g / kg, has the advantages of being convenient and rapid to operate, high in sensitivity and repeatability and accurate in quantitation and quantification, can meet the technical requirements of the corresponding product safety inspection of nations such as the U.S., Japan, the European Union and Canada, and provides powerful technical support for food safety of people and healthy development of export trade in China.

Description

technical field [0001] The invention relates to a method for determining the residual amount of chlorantraniliprole in fruits and vegetables, more specifically, the qualitative and quantitative determination of residual chlorine in vegetables and fruits by gas chromatography-negative chemical ion source-mass spectrometry (GC-NCI-MS) The invention discloses a method for the content of benzamide, which belongs to the technical field of determination of pesticide residues. Background technique [0002] Chlorantraniliprole (Chlorantraniliprole), trade name Kang Kuan, is a new type of o-formamidobenzamide insecticide developed by DuPont. Mothidae, Fruit Mothidae, Leaf Rolleridae, Melydidae, Plutella Plutella, Graciidae, Thymothidae, etc. all have very good control effects, and can also control Coleoptera Weevilidae and Chromophyidae; Muscidae; Bemisia tabaci and other non-Lepidoptera pests. The chemical name is: 3-bromo-N-[4-chloro-2-methyl-6-[(methylcarbamoyl)benzene]-1-(3-chl...

AI Technical Summary

Problems solved by technology

Now various testing institutions and enterprises have purchased gas chromatography-mass spectrometers (GC-MS), and are generally equipped with negative chemical ion sources (NCI). Now many types of pesticides contain electronegative groups, organochlorines and pseudo Pyrethrin pesticide molecules mostly contain strong electronegative groups such as -F, -Cl, -Br or -COO-; organophosphorus pesticide molecules mostly contain =S, -OR, -P, -O-, -Cl, or -P=O and other electronegative groups; and most of the new pesticides developed in recent years contain -F groups. Therefore, the use of GC-NCI-MS can facilitate the multi-residue analysis of various pesticides. Compared with MS, it can obtain better anti-interference ability, lower sensitivity and better selectivity. Chlorantraniliprole is an electronegative compound, but so far no chlorantraniliprole residues have been seen in vegetables and fruits The report of the GC-NCI-MS detection method of amount, therefore, the detection method of establishing gas chromatography-negative chemical ion source-mass spectrometry (GC-NCI-MS) qualitative and quantitative analysis of chlorantraniliprole residues in vegetables and fruits has important meaning

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