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Trace detection method for pharmacodynamic active ingredients of Sinkiang fritillary bulb and pharmacodynamic active ingredients thereof

An active ingredient, tracking detection technology, applied in the direction of organic active ingredients, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve the problem of weak activity, unsuitable for iberecium, etc., and achieve the effect of accurate and comprehensive tracking and detection

Inactive Publication Date: 2015-03-25
广东通仁药业有限公司
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Problems solved by technology

[0005] And the inventor has just found similar situation in the research process of the active ingredient of Iglobulina. The traditional Chinese medicine Ignebomu has a strong cough-relieving curative effect, but the main monomer substance sibetin obtained by phytochemical separation is more effective than Igbemula. The activity of the total extract is much weaker, which means that the traditional method of separating the chemical components of traditional Chinese medicine one by one and then tracking the active components is not suitable for Yibeimu

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  • Trace detection method for pharmacodynamic active ingredients of Sinkiang fritillary bulb and pharmacodynamic active ingredients thereof
  • Trace detection method for pharmacodynamic active ingredients of Sinkiang fritillary bulb and pharmacodynamic active ingredients thereof
  • Trace detection method for pharmacodynamic active ingredients of Sinkiang fritillary bulb and pharmacodynamic active ingredients thereof

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Embodiment

[0041] A method for tracking and detecting medicinally active ingredients of Yibeimu, comprising the following steps:

[0042] 1) Extraction of components: extracting various components in Igbomu to obtain sibetin and total flavonoids of Igbomu.

[0043] 1. The preparation method of sibetin: pulverize the ibeimu, add 95% ethanol by volume percentage, reflux extraction for 3 hours, extract 3 times, filter with suction, merge the filtrate, reclaim the ethanol under reduced pressure, obtain the thick extract, go to Add distilled water to the thick extract to suspend the thick extract, then use 1mol / L NaOH to adjust the pH value to 10, extract the lye with chloroform three times, combine the extracts, and dry them in vacuum to obtain the total alkaloids of Igbomu. Purified by chromatography to obtain sibetin (using UV, MS, 1 H and 13 Spectral methods such as C NMR have carried out the characterization to this product, confirm that it is sibecin).

[0044] 2. The preparation met...

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Abstract

The invention discloses a trace detection method for pharmacodynamic active ingredients of Sinkiang fritillary bulb and pharmacodynamic active ingredients of Sinkiang fritillary bulb and belongs to the technical field of screening of pharmacodynamic active ingredients. The method comprises the following steps: (1) extracting the components, namely extracting various components in the Sinkiang fritillary bulb; (2) matching the multiple components, namely combining all the obtained components, thereby obtaining at least one composition; (3) measuring an oil and water distribution coefficient, namely respectively measuring the oil and water distribution coefficients of the various components and the components in the composition; and (4) tracing the active ingredients, namely by taking the obtained oil and water distribution coefficients as activity evaluation indexes, performing contrast detection on a standard oil and water distribution coefficient. According to the method, the in-vivo absorption and distribution conditions of the active ingredients are considered, the interaction among the pharmaceutical active ingredients is also considered, and therefore the method is an excellent trace detection method for the pharmacodynamic active ingredients.

Description

technical field [0001] The invention relates to a screening method for active ingredients of medicines, in particular to a method for tracking and detecting the active ingredients of ibamma and its active ingredients. Background technique [0002] Traditional Chinese medicine is the product of clinical practice of traditional Chinese medicine for thousands of years, and its curative effect is unquestionable. However, due to the diversity of traditional Chinese medicine components and the complexity of the mechanism of action, research on the material basis of traditional Chinese medicine efficacy has always been a hot and difficult research topic for researchers. [0003] So far, the basic research on pharmacodynamic substances of traditional Chinese medicine has mainly used the mode of step-by-step separation of phytochemicals and tracer of pharmacological activity. This research model has achieved certain results. However, during the research process, it was also found tha...

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Application Information

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IPC IPC(8): G01N30/02A61K36/8966A61K31/4375
Inventor 焦豪妍汤庆发张现涛付晓春杨燕军沈小钟聂阳
Owner 广东通仁药业有限公司
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