Method for forcing dunaliella tertiolecta to accumulate beta-carotene

Abstract

The invention discloses a method for forcing dunaliella tertiolecta to accumulate beta-carotene and belongs to the aquaculture field. Conventionally, the dunaliella tertiolecta adopts an open type runway big pool and uses an Hutner culture solution to culture, is slow in growth, low yin yield, easy to pollute, high in cost and low in content of beta-carotene, and the illumination intensity and the temperature are completely dependent on nature. In order to overcome the defects in the prior art, indoor light-control temperature-control culture is adopted, the illumination intensity and the temperature are regulated according to the special needs of different growth stages of the dunaliella tertiolecta, especially at the later culture period, the illumination intensity and the temperature are increased, ammonium chloride, human urine, calcium phosphate, thiamine hydrochloride and the like are supplemented, the conventional bath culture method is changed; after the later culture period, nitrogen salt and phosphor salt in the culture solution are removed, the salinity is improved, the growth speed of the dunaliella tertiolecta is greatly increased, and the content of the beta-carotene is increased by 70%.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a method for stressing Dunaliella salina to accumulate beta-carotene. Background technique [0002] Dunaliella salina is a diflagellated unicellular green alga that can tolerate high salinity (20-300‰). Dunaliella salina can accumulate a large amount of β-carotene under stress conditions, up to 14% of dry weight, which is the first of all organisms in nature. Therefore, as early as 1966, Massyuk proposed that Dunaliella salina can be cultivated on a large scale to produce β-carotene; in addition, the glycerol contained in Dunaliella salina can reach 40% of the dry weight, which has the potential for producing glycerol; Dunaliella salina is also a good feed for aquatic animals. my country has a long coastline and numerous inland salt lakes, and has unique natural conditions for cultivating Dunaliella salina to produce β-carotene. [0003] The types and proportions of nitro...

AI Technical Summary

Problems solved by technology

[0005] Traditionally, the large-scale cultivation of Dunaliella salina adopts open raceway-type large ponds and uses Hutner culture medium for cultivation. The light intensity and temperature are completely dependent on nature, which is erratic and does not conform to the growth of Dunaliella salina in each stage and the accumulation of β-carotene. Requirements, uncontrollable, slow growth, low output, easy to pollute, high cost, large basic investment, low β-carotene content, poor product quality

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