Method for fast separating and extracting polypeptide and polysaccharide from corbicula fluminea

Abstract

The invention relates to a method for fast separating and extracting polypeptide and polysaccharide from corbicula fluminea. The method comprises the following steps: carrying out mashing pretreatment on corbicula fluminea meat; adding Alcalase alkaline protease of 1.5%-2.5% to a corbicula fluminea liquid with protein content of 1%-2%, regulating pH to 8-9, carrying out enzymolysis at 45-55 DEG C for 3 hours, carrying out enzyme deactivation at 90 DEG C for 10-15 minutes, and centrifugalizing to take supernate; separating the polypeptide through a macroporous adsorption resin column, loading a sample at the flow velocity of 1-2 BV / h and the concentration of 10-30 mg / ml, washing at the velocity of 2-4 BV / h, then resolving at the flow velocity of 1-3 ml / min by using ethanol, and carrying out rotary evaporation and concentration and freeze drying on a resolved solution to obtain corbicula fluminea polypeptide powder; adding a washing solution obtained from the last step to an ion-exchange chromatography column, respectively enabling an NaCl solution of 0.1-2 mol / L and deionized water to pass through the ion-exchange chromatography column at the flow velocity of 1-3 ml / min, collecting in steps, mixing same components, carrying out the rotary evaporation and concentration, dialyzing by using ionized water, carrying out the freeze drying on a dialyzed solution to obtain corbicula fluminea polysaccharide dry powder. The method disclosed by the invention is easy to operate and can be used for extracting the polypeptide and the polysaccharide at the same time and solving the problem that the polysaccharide is wasted when the polypeptide is extracted or the polypeptide is wasted when the polysaccharide is extracted. The polypeptide and the polysaccharide are high in yield and both have very good physicochemical property and functional property.

Description

technical field [0001] The invention belongs to the technical field of biological extraction and relates to a method for extracting polypeptide polysaccharides. Background technique [0002] River clams are widely found in waters all over the world. It has fast growth speed, strong reproductive ability, large breeding output, rich nutrition, delicious taste, high edible value and health care function. , measles, fever, cough and phlegm and hangover and other effects. However, for the current utilization of river clam resources, although products such as concentrated clam juice and clam essence (produced in Taiwan) have been developed, most of them are still eaten fresh or made into dried clams, salted clams, canned products, etc. Mainly, the deep processing rate is still low, resulting in its economic value not being fully utilized. [0003] The traditional method of separating and extracting polypeptide polysaccharides is often to obtain polypeptides, which destroys the ...

AI Technical Summary

Problems solved by technology

However, for the current utilization of river clam resources, although products such as concentrated clam juice and clam essence (produced in Taiwan) have been developed, most of them are still eaten fresh or made into dried clams, salted clams, canned products, etc. Mainly, the deep processing rate is still low, resulting in its economic value not being fully utilized

[0003] The traditional method of separating and extracting polypeptide polysaccharides is often to obtain polypeptides, which destroys the structure and activity of polysaccharides during processing, resulting in waste of active polysaccharides; or in order to obtain polysaccharides, use Sevag method, trifluorotrichloroethane method, Deproteinization by methods such as trichloroacetic acid method leads to waste of protein