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Micro algae-biochar composite bio-fertilizer and preparation method thereof

A technology of compound biological fertilizer and biochar is applied in the directions of organic fertilizers, fertilization devices, fertilizer mixtures, etc., which can solve the problems of increasing soil fertility, low mineral nutrient content, and high processing costs, so as to improve soil fertility and plant yield, increase The effect of soil available phosphorus content and improving soil enzyme activity

Active Publication Date: 2015-03-25
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods can alleviate the problem of soil degradation to a certain extent, but there are still the following disadvantages: (a) physical remediation technology cannot fundamentally improve soil fertility, and the related equipment is expensive and the treatment cost is too high; (b) chemical remediation only It can change the properties of degraded soil in a short time, and the general chemical restoration technology needs water, so the restoration site needs to be close to the water source, and at the same time, the cost is increased due to the need to treat wastewater; (c) bioremediation can increase the content of organic matter and reduce the concentration of saline-alkali soil salinity and alkalinity, but the restoration period is long, monitoring and management are difficult, and the land after restoration can only be used as a green belt
Biochar itself is rich in organic carbon, but its mineral nutrient content is usually low, and its supplementary effect on soil nutrients is not as obvious as that of general fertilizers, and there are large differences in the mineral nutrient content of different biochar materials
Although biochar itself can be directly applied to the soil to improve soil fertility and increase crop yield, because the C / N of biochar is higher than that of other organic matter, its transformation in the soil requires a large amount of N, which seriously affects and restricts Soil N supply
When biochar is applied to the soil, it competes with crops for N, reducing N availability
Moreover, when biochar is directly applied to farmland, it will take a certain amount of time to achieve the effect. Driven by the reality of pursuing increased grain production and improving efficiency, it is still difficult to rely solely on biochar to increase soil fertility or improve soil.

Method used

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  • Micro algae-biochar composite bio-fertilizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Microalgae-biochar-based fertilizer supplemented with crop waste to improve sandy / desertified soil

[0032] Microtheca was isolated and purified from sandy soil crusts by BG11 nitrogen-deficient medium plate method several times. In a 60L photobioreactor (1%CO 2 , LED light intensity 60μmol photon m -2 ·s -1 , 25°C) for 7 days of scale-up culture with the exclusive medium prepared above to screen and cultivate microalgal species with high nitrogen-fixing capacity and high carbon-fixing capacity. After the biomass reaches 0.9g / L, the microalgae are enriched by gravity sedimentation and scaled up. Then, use the new thin layer (RW) drying technology to collect active microalgae, and prepare compound biofertilizer according to the mass ratio of microalgae:biochar:crop waste equal to 15:35:50.

[0033] After applying the compound biological fertilizer, the following effects are achieved:

[0034] 1. It can effectively improve the aggregate structure of sandy s...

Embodiment 2

[0039] Example 2 Microalgae-biochar base fertilizer supplemented with organic fertilizer and quick-acting fertilizer to improve compacted or barren soil

[0040]Nostoc was isolated and purified several times from hardened soil crusts by BG11 nitrogen-deficient medium plate method. In a 60L photobioreactor (2%CO 2 , LED light intensity 100μmol photon m -2 ·s -1 , 25°C), use the above-mentioned special medium prepared above which can represent the floor junction or barren soil environment to carry out amplified culture for 7 days to screen and cultivate microalgal species with high nitrogen fixation capacity and high carbon fixation capacity. After the biomass reached 1.2g / L, the microalgae were enriched by filtration and carried out on a large scale, and then the microalgae were treated overnight by vacuum freeze-drying technology to keep the microalgae active. Finally, active microalgae are collected, and compound biofertilizer is prepared according to the mass ratio of mic...

Embodiment 3

[0048] Example 3 Microalgae-biochar-based fertilizer supplemented with alkaline slag to improve acidified soil

[0049] Anabaena was isolated and purified from acidified soil crusts by BG11 nitrogen-deficient medium plate method. In a 60L photobioreactor (1.5%CO 2 , LED light intensity 60μmol photon m -2 ·s -1 , 25°C), use the above-mentioned special culture medium that can represent the acidified soil environment of the territory to carry out amplified culture for 7 days to screen and cultivate microalgal species with high nitrogen fixation capacity and high carbon fixation capacity. After the biomass reached 1.0g / L, the microalgae were enriched by centrifugation at 4000×g, and then cultured on a large scale. Then, spray drying is performed to keep the microalgae alive. Finally, collect microalgae, according to microalgae: biochar: alkaline slag (pH value 9-12, the CaCl that contains mass ratio 35%) 2 , 40% CaCO 3 , 25% CaSO 4 ): The mass ratio of crop waste equal to 1...

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Abstract

The invention discloses a preparation method of a micro algae-biochar composite bio-fertilizer. The method comprises the following steps: (1) separating representative photosynthetic microorganisms in soil crust, and preparing an exclusive culture medium; (2) screening and cultivating algae species having high nitrogen fixation ability and high carbon fixation ability by virtue of the exclusive culture medium in a photobioreactor; (3) enriching microalgae; (4) carrying out large-scale cultivation of microalgae; (5) collecting active microalgae; (6) collecting the biochar which is obtained by biomass pyrolysis, and carrying out drying treatment; and (7) compounding the active microalgae with the biochar, so as to prepare the micro algae-biochar composite bio-fertilizer. The invention also discloses a bio-fertilizer prepared by the method. The micro algae-biochar composite bio-fertilizer is prepared from the microalgae with high nitrogen fixation ability and pyrolysis biochar rich in high organic carbon as main matrixes; the advantages of the nitrogen-fixing microalgae and the biochar can be put into a full play; effective improvement and repair of the soil are achieved; and development of green low-carbon agriculture is promoted.

Description

technical field [0001] The invention relates to the biological field, in particular to a bio-based compound organic fertilizer and a production method thereof. Background technique [0002] Soil is the material basis for maintaining the biomass of the earth's surface ecosystem, and it is an inseparable natural resource and environment. At the same time, it also provides humans with various agricultural, forestry and pharmaceutical raw materials including food. However, with the continuous development of modern agriculture characterized by intensive utilization in our country, the threats to land resources, environment and human survival are becoming increasingly apparent. The main feature of intensive modern agriculture is to ensure and increase the yield of food crops through the high input of agrochemicals (mainly pesticides and fertilizers, etc.). The total amount of chemical fertilizers and pesticides used in my country ranks first in the world, and the amount used per ...

Claims

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Application Information

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IPC IPC(8): C05F11/08
CPCC05F11/00C05G3/80
Inventor 孙予罕非罗科赵权宇胡刚范卫尧孙鼎堃肖亚宁王东飞
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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