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Method for preparing sugarcane sheath silage by using lactobacillus buchneri, candida tropicalis and bacillus subtilis

A technology of Candida tropicalis and Bacillus subtilis, which is applied in the field of silage preparation, can solve problems such as no reports, and achieve the effects of easy operation conditions, easy availability of raw materials, and simple preparation method.

Inactive Publication Date: 2015-03-25
GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, so far, there have been no reports in the literature on the combined application of Lactobacillus brunii, Candida tropicalis and Bacillus subtilis to silage fresh sugarcane tails.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The method for preparing sugarcane tail silage with Lactobacillus Brucelli, Candida tropicalis and Bacillus subtilis, the operation steps are as follows:

[0029] 1. Collection of silage raw materials:

[0030] Fresh sugarcane tails after sugarcane harvesting are collected as raw material.

[0031] 2. Chopped:

[0032] Cut the fresh sugarcane tails into 1cm-4cm lengths respectively.

[0033] 3. Add evenly 0.5% of fresh weight urea to the chopped fresh sugarcane tails in step 2.

[0034] 4. Add the mixed preparation of Lactobacillus Brucella, Candida tropicalis and Bacillus subtilis.

[0035] Spray the mixed preparation of Lactobacillus Brucella, Candida tropicalis and Bacillus subtilis evenly on the fresh sugarcane tail raw material after adding urea in step 3, so that the fresh sugarcane tails contain Lactobacillus Brucella, Candida tropicalis and Bacillus subtilis The number of viable bacteria reached 2×10 6 cfu / g, 2×10 5 cfu / g, 7×10 6 cfu / g.

[0036] 5. Filli...

Embodiment 2

[0045] The method for preparing sugarcane tail silage with Lactobacillus Brucelli, Candida tropicalis and Bacillus subtilis, the operation steps are as follows:

[0046] 1. Collection of silage raw materials:

[0047] Fresh sugarcane tails after sugarcane harvesting are collected as raw material.

[0048] 2. Chopped:

[0049] Cut the fresh sugarcane tails into 1cm-4cm lengths respectively.

[0050] 3. Add evenly 0.5% of fresh weight urea to the chopped fresh sugarcane tails in step 2.

[0051] 4. Add the mixed preparation of Lactobacillus Brucella, Candida tropicalis and Bacillus subtilis:

[0052] Spray the mixed preparation of Lactobacillus Brucella, Candida tropicalis and Bacillus subtilis evenly on the fresh sugarcane tail raw material after adding urea in step 3, so that the fresh sugarcane tails contain Lactobacillus Brucella, Candida tropicalis and Bacillus subtilis The number of viable bacteria reached 4×10 6 cfu / g, 4×10 5 cfu / g, 1.4×10 7 cfu / g.

[0053] 5. Fil...

Embodiment 3

[0061] The method for preparing sugarcane tail silage with Lactobacillus Brucelli, Candida tropicalis and Bacillus subtilis, the operation steps are as follows:

[0062] 1. Collection of silage raw materials:

[0063] Fresh sugarcane tails after sugarcane harvesting are collected as raw material.

[0064] 2. Chopped:

[0065] Cut the fresh sugarcane tails into 1cm-4cm lengths respectively.

[0066] 3. Add evenly 0.5% of fresh weight urea to the chopped fresh sugarcane tails in step 2.

[0067] 4. Add the mixed preparation of Lactobacillus Brucella, Candida tropicalis and Bacillus subtilis:

[0068]Spray the mixed preparation of Lactobacillus Brucella, Candida tropicalis and Bacillus subtilis evenly on the fresh sugarcane tail raw material after adding urea in step 3, so that the fresh sugarcane tails contain Lactobacillus Brucella, Candida tropicalis and Bacillus subtilis The number of viable bacteria reached 6×10 6 cfu / g, 6×10 5 cfu / g, 2.1×10 7 cfu / g.

[0069] 5. Fill...

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PUM

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Abstract

The invention discloses a method for preparing sugarcane sheath silage by using lactobacillus buchneri, candida tropicalis and bacillus subtilis. The method disclosed by the invention comprises the following steps: cutting short the mowed sugarcane sheaths to achieve a length of 1-4cm, uniformly spraying the mixed preparation of lactobacillus buchneri, candida tropicalis and bacillus subtilis to the fresh sugarcane sheath raw materials added with 0.5% of urea to enable the viable counts of lactobacillus buchneri, candida tropicalis and bacillus subtilis contained in the fresh sugarcane sheaths to achieve 2*10<6>cfu / g to 6*10<6>cfu / g, 2*10<5>cfu / g to 6*10<5>cfu / g and 7*10<6>cfu / g to 2.1*10<7>cfu / g respectively, uniformly mixing, filling, compacting, sealing to achieve a density of 0.4kg / L, and storing for 40 days at room temperature, so as to prepare the sugarcane sheath silage. The method disclosed by the invention solves the problem of poor palatability caused by the fact that sugarcane sheaths are directly fed to ruminant animals, and can increase the content of crude proteins in the sugarcane sheath silage.

Description

technical field [0001] The invention belongs to the technical field of silage preparation, and in particular relates to a method for preparing sugarcane tail silage by using Lactobacillus Brucella, Candida tropicalis and Bacillus subtilis. Background technique [0002] The harvest of sugarcane is seasonal. According to reports, the output of Guangxi alone in the 2012-2013 crushing season exceeded 75 million tons, and the output of sugarcane tail accounted for about 1 / 4 of the sugarcane output, which was 18.75 million tons. The problems encountered by sugarcane tails as forage grass for ruminants are that the palatability of direct application of sugarcane tails is poor, and the second is that the harvest season of sugarcane tails coincides with winter, and it is not easy to make dry forage grass. Therefore, can these sugarcane tails Effective treatment and long-term preservation are of great significance to the development of animal husbandry. Silage production is an import...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K3/02A23K1/14A23K1/00
Inventor 邹彩霞梁辛夏中生韦升菊唐振华梁贤威李丽莉林波黄锋杨承剑唐庆凤覃广胜莫乃国赵朝步潘玉红
Owner GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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