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Schisandra chinensis extract and application thereof in bacteriostasis

A technology of Schisandra chinensis extract and Schisandra chinensis, which can be used in applications, chemicals for biological control, fungicides, etc., and can solve problems such as few reports.

Active Publication Date: 2015-03-25
北京汇德荣生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, Schisandra chinensis has the potential to be developed as a safe new type of pesticide, but there are not many reports on this aspect

Method used

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  • Schisandra chinensis extract and application thereof in bacteriostasis
  • Schisandra chinensis extract and application thereof in bacteriostasis
  • Schisandra chinensis extract and application thereof in bacteriostasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The preparation of embodiment 1, Schisandra chinensis extract

[0052] 1. Raw materials: dried stems and leaves of Schisandra chinensis;

[0053] 2. Configure organic solutions with different concentrations:

[0054] (1) Organic solution A is made up of chloroform;

[0055] (2) Organic solution B is made up of chloroform and acetone, wherein the volume ratio of chloroform and acetone=10:1;

[0056] (3) The organic solution C is composed of chloroform and acetone, wherein the volume ratio of chloroform and acetone=9:1;

[0057] (4) The organic solution D is composed of chloroform and acetone, wherein the volume ratio of chloroform and acetone=2:1;

[0058] (5) The organic solution E is composed of chloroform and acetone, wherein the volume ratio of chloroform and acetone=1:1;

[0059] (6) Organic solution F is made up of acetone;

[0060] (7) The organic solution G consists of methanol.

[0061] 3. Preparation of Schisandra chinensis extract:

[0062] 10kg of drie...

Embodiment 2

[0071] Embodiment 2, the application of Schisandra chinensis extract in inhibiting fungal growth

[0072] 1. Experimental materials and methods

[0073] 1. Preparation of pathogen spore suspension

[0074] Peach brown rot fungus (Monilinia fructicola), which can cause peach brown rot (Yang Jinfeng, Li He, Liu Suhua, Hu Haiwen, Liu Zhengping, Zhao Xiaoyan. The antibacterial effect of three traditional Chinese medicine extracts on peach brown rot fungus (Monilinia fructicola), China Agronomy Bulletin 2009,25(12):188-194);

[0075] Apple ring spot caused by Botryosphaeria berengeriana f.sp.piricola (Dong Juanhua, Li Baohua. Research progress on apple ring spot, Northern Fruit Tree, 2009, 9(1): 1-2 );

[0076] Fusarium oxysporum can cause strawberry root rot (Wang Zhongwu, Zang Huiming. Pathogen identification and biological characteristics of strawberry root rot, Guangdong Agricultural Science, 2011 (8): p.);

[0077] Pathogenic bacteria were cultivated with fresh PDA medium....

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PUM

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Abstract

The invention discloses a schisandra chinensis extract and an application thereof in bacteriostasis, and provides a preparation method of the schisandra chinensis extract. The preparation method comprises the following steps: soaking schisandra chinensis stems and leaves with a 70% acetone / aqueous solution; extracting with ethyl acetate, carrying out column chromatography on silica gel, and carrying out gradient eluting with chloroform / acetone (1:0 to 0:1), wherein the volume ratio of chloroform to acetone is equal to 10:0, 10:1 and 9:1. An experiment proves that the extraction effects of different ratios of organic solvents on schisandra chinensis fruits are groped; the extract with the ratio of organic solvent chloroform to acetone being 9:1 displays a strong inhibiting effect on three harmful fungi; and a basis is provided for further development of environment-friendly bactericides.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Schisandra chinensis extract and its application in antibacterial. Background technique [0002] The use of chemical pesticides has a history of nearly a hundred years. For a long time, chemical pesticides have greatly protected agricultural production and harvest, and have played an irreplaceable role in the development and progress of human beings and society. Although traditional chemical pesticides have a positive effect on increasing agricultural production and preventing pests and diseases, they also have serious negative effects, destroying ecological balance, and seriously polluting the environment: such as residues that seriously reduce the quality of crops, accumulation of harmful ingredients, and endanger human health ; All kinds of diseases and insect pests generally produce strong drug resistance, which makes the prevention and control of diseases and insect pests more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/08A01P3/00
Inventor 陈艳肖伟烈刘俊姚允聪伊海静石一鸣魏朝俊庞金环
Owner 北京汇德荣生物技术有限公司
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