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In-vitro rapid propagation technique of melaleuca alternifolia

An in vitro technology of Melaleuca alternifolia, applied in the field of plant tissue culture, can solve the problems of underdeveloped seeds, lack of high-quality seedlings, and low germination rate of seeds, so as to save cost and space, improve the efficiency of rapid propagation, shorten The effect of the production cycle

Inactive Publication Date: 2015-03-25
杨业容
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Problems solved by technology

Because the seeds of Melaleuca alternifolia are underdeveloped, they are often aborted, and the germination rate of seeds is low, resulting in the lack of good seedlings, which restricts the popularization and planting of Melaleuca alternifolia

Method used

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Embodiment Construction

[0016] The following examples are to further illustrate the present invention, but not to limit the present invention.

[0017] Specific implementation

[0018] (1) Disinfection and primary culture of explants: select the young stems of healthy and disease-free saplings as explants, rinse them in tap water for 4 hours, and soak them in 75% ethanol for 10 seconds in an ultra-clean workbench , 0.1% mercury liter solution disinfected for 20 minutes, and then washed with sterile water 3 times, wiped dry and cut into stems with a length of 0.5 ~ 1.2 cm, inoculated on the primary medium, first cultivated in the dark at 28 ℃ for 20 days , And then placed in the light for 10 hours a day, the light intensity is 1500lx, and the culture temperature is 25-28 ℃ for 45 days to form adventitious buds. The primary culture medium is: MS2mg / L6-BA+1mg / L NAA+3.5% sucrose+0.5% agar+0.05% activated carbon+0.01mmol / L La(NO 3 ) 2 , The pH is 5.8

[0019] (2) Proliferation culture: The adventitiou...

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Abstract

The invention relates to an in-vitro rapid propagation technique of melaleuca alternifolia and relates to a seeding raising method for obtaining high-quality seedlings of melaleuca alternifolia by virtue of in-vitro rapid propagation. The method is characterized in that tender stem segments of melaleuca alternifolia are adopted as explants, and the in-vitro rapid propagation of the melaleuca alternifolia can be realized through the procedures such as selection and sterilization of the explants, primary culture, multiplication culture, in-bottle rooting, acclimatization and transplanting, and the like. An important significance for satisfying the demand of the melaleuca alternifolia seedlings and accelerating the popularization of the improved variety of the melaleuca alternifolia can be realized.

Description

Technical field [0001] The invention relates to a method for culturing plant tissues in agricultural biotechnology, in particular to a technique for in vitro rapid propagation of Melaleuca alterniflora. Background technique [0002] Melaleuca alterniflora ( Melaleuca ahemifolia), also known as tea oil tree, is a spice plant in the genus Melaleuca in the Myrtle family. Melaleuca alterniflora is mainly produced on the northern coast of New South Wales, Australia. It is called Australian gold and is a famous magical skin treatment product in Australian aboriginal legends. Its branches and leaves are rich in aromatic oils, namely Tea Tree Oil, which is widely used in the spice seasoning industry, perfume, and cosmetics industry. [0003] my country has introduced Melaleuca alterniflora in Guangxi, Guangdong, Fujian and other places since the 1990s. Because the seeds of Melaleuca alterniflora are underdeveloped, often aborted, and the germination rate of seeds is low, the lack of...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨业容
Owner 杨业容
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