Aspergillus oryzae functional enzyme preparation and application thereof
An Aspergillus oryzae and enzyme preparation technology, which is applied in the field of Aspergillus oryzae functional enzyme preparations, can solve the problems of limiting the taste of soft and soft liquor, microbial enrichment cannot fully meet the needs of winemaking production, limited types and quantities of microorganisms, etc. and enrichment, realize automatic and mechanized production, and improve the quality of raw wine
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Embodiment 1
[0034] Preparation of Aspergillus oryzae enzyme preparation:
[0035] ①Aspergillus oryzae solid-state strains: use rice koji juice agar slant as the medium, inoculate 1 ring of the scientific name Aspergillus oryzaeAs3.042 strain, culture at 28°C for 72 hours; take bran, add water according to the mass ratio of bran:water=1:1 Stir evenly, sterilize at 121°C and 0.1MPa for 0.5h, cool to 35°C for inoculation, and culture at 32°C for 72h to obtain solid Aspergillus oryzae strains;
[0036] ②Moist Aspergillus oryzae functional enzyme preparation: Take the bran, add water according to the mass ratio of bran:water=5:3, autoclave and cool to 32°C, insert the solid Aspergillus oryzae at 1% by mass, mix well and spread out , humidity 85%, control product temperature 32 ℃, culture 72h;
[0037] ③Drying: put the wet functional enzyme preparation of Aspergillus oryzae into a drying room with a thickness of 1 cm for drying treatment, the drying temperature is 40° C., and the drying time i...
Embodiment 2
[0045] Preparation of Aspergillus oryzae enzyme preparation:
[0046] ① Aspergillus oryzae solid-state strains: use rice koji juice agar slant as medium, inoculate 1 and a half rings of Aspergillus oryzaeAs 3.042 strain, and cultivate at 30°C for 72 hours; take bran, add water according to the mass ratio of bran:water=3:1 Stir evenly, sterilize at 121°C and 0.1MPa for 0.5h, cool to 40°C for inoculation, and culture at 40°C for 48h to obtain solid Aspergillus oryzae strains;
[0047] ②Moist Aspergillus oryzae functional enzyme preparation: Take the bran, add water according to the mass ratio of bran:water=3:1, autoclave and cool to 40°C, insert the solid Aspergillus oryzae at 8% by mass, mix well and spread out , control the product temperature at 40°C, and cultivate for 48 hours;
[0048] ③Drying: put the wet functional enzyme preparation of Aspergillus oryzae into a drying room with a thickness of 1.5 cm for drying treatment, the drying temperature is 40°C, and the drying ti...
Embodiment 3
[0056] Preparation of Aspergillus oryzae enzyme preparation:
[0057] ① Aspergillus oryzae solid-state strains: use the rice koji juice agar slant as the medium, inoculate 2 rings of Aspergillus oryzae As3.042 strain, and culture at 32°C for 72 hours; take bran, according to the mass ratio of bran:water=2:1 Add water and stir evenly, sterilize at 121°C﹑0.1MPa for 0.5h, cool to 20°C for inoculation, and culture at 20°C for 24h to obtain solid Aspergillus oryzae strains;
[0058] ②Moist Aspergillus oryzae functional enzyme preparation: Take the bran, add water according to the mass ratio of bran:water=1:1, autoclave and cool to 20°C, insert the solid Aspergillus oryzae at 15% by mass, mix and spread evenly , humidity 85%, control product temperature 20 ℃, culture 24h;
[0059] ③Drying: put the wet Aspergillus oryzae functional enzyme preparation in a drying room with a thickness of 2 cm for drying treatment, the drying temperature is 80° C., and the drying time is 12 hours to o...
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