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A kind of mulberry leaf polysaccharide and its separation and purification preparation method and application

A technology for the separation and purification of mulberry leaf polysaccharides, which is applied in blood diseases, extracellular fluid diseases, drug combinations, etc., can solve the problems that there are no reports on fractional separation, purification and property research of mulberry leaf polysaccharides, and achieve good anticoagulant function, The effect of low energy consumption and high coagulation activity

Active Publication Date: 2016-09-07
ANHUI POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These reports have greatly enriched the extraction, purification and biological activity of mulberry polysaccharides, but there is no report on the further fractionation, purification and properties of mulberry polysaccharides.

Method used

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  • A kind of mulberry leaf polysaccharide and its separation and purification preparation method and application
  • A kind of mulberry leaf polysaccharide and its separation and purification preparation method and application
  • A kind of mulberry leaf polysaccharide and its separation and purification preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] After the mulberry leaves are pulverized by a pulverizer, pass through a 100-mesh sieve to obtain the mulberry leaf coarse powder, extract the mulberry leaf coarse powder and distilled water at a weight ratio of 1:10 at 60°C for 90 minutes, remove impurities to obtain the filtrate A, and concentrate the filtrate A to 1 / 5 of the original volume, then slowly add 4 times the volume of absolute ethanol of the concentrated filtrate A until the volume concentration of ethanol reaches 80%, centrifuge to remove the supernatant after alcohol precipitation for 12 hours, and vacuum freeze-dry to obtain the crude polysaccharide of mulberry leaves a.

[0053] Dissolve the prepared mulberry leaf crude polysaccharide A in distilled water, centrifuge for 10 minutes at a speed of 4000r / min, and then put it on a polyamide column, use distilled water as the mobile phase, and the flow rate is 1.0mL / min, collect the polysaccharide solution, and collect the polysaccharide The pH of the solut...

Embodiment 2

[0057] After the mulberry leaves are pulverized by a pulverizer, pass through a 60-mesh sieve to obtain the mulberry leaf coarse powder, extract the mulberry leaf coarse powder and distilled water at a weight ratio of 1:40 at 90°C for 150 minutes, remove impurities to obtain the filtrate A, and concentrate the filtrate A to 1 / 5 of the original volume, then slowly add 4 times the volume of dehydrated ethanol of the concentrated filtrate A until the volume concentration of ethanol reaches 80%, centrifuge to remove the supernatant after alcohol precipitation for 24 hours, and vacuum freeze-dry to obtain the crude polysaccharide of mulberry leaves a.

[0058] Dissolve the prepared mulberry leaf crude polysaccharide A in distilled water, centrifuge for 20min at a speed of 3600r / min, and then put it on a polyamide column, use distilled water as the mobile phase, and the flow rate is 2.0ml / min, collect the polysaccharide solution, and collect the polysaccharide The pH of the solution...

Embodiment 3

[0061] Embodiment 3 anticoagulant health product—preparation of mulberry leaf drink:

[0062] In parts by weight, the ratio of water to coarse mulberry leaves is 1-20:100, the extraction temperature is 75-95°C, and the time is 5-30 minutes. After leaching, use 250-mesh nylon cloth to filter and remove residue, and quickly cool to 5°C. , placed for 24h to clarify, 0.5μm fine filtration to obtain clarified mulberry leaf juice. 0.04-0.2% MPS-2b, 3-6% sucrose, 0.03-0.07% ascorbic acid and other known excipients are firstly dissolved in some warm water and filtered. Mix it with the finely filtered mulberry leaf juice, sterilize at 135° C. for 5-7 seconds, and bottle it to obtain an anticoagulant health-care mulberry leaf drink.

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Abstract

The invention relates to folium mori polysaccharide, a separation and purification preparation method thereof and an application of folium mori polysaccharide in preparation of anticoagulant healthcare products and medicine. The preparation method of folium mori polysaccharide comprises following steps: folium mori is subjected to smashing, aqueous extraction and alcohol precipitation, passes a polyamide column for deproteinization, is subjected to H2O2 decoloration and then is subjected to separation and purification by a DEAE-sepharose CL-6B ion exchange column and a sepharose CL-6B gel column to obtain folium mori polysaccharide. Further classified separation and purification of folium mori polysaccharide are realized, a pure product of folium mori polysaccharide with an average molecular weight of (4.5-8.0)*10<4>Da is obtained, and experiments prove that folium mori polysaccharide has high coagulation activity, is safe and non-toxic, and can be developed with other raw materials and auxiliary materials which are acceptable in healthcare product and medicine processes to form healthcare products and medicine with the good anticoagulant function, so that folium mori polysaccharide has good development and application prospect.

Description

technical field [0001] The invention relates to a separation and purification preparation method of mulberry leaf polysaccharide and the application of the mulberry leaf polysaccharide in medicine and health products. Background technique [0002] With the improvement of people's living standards and changes in diet structure, thrombotic disease is the most common cardiovascular and cerebrovascular disease, which seriously threatens people's health. According to the WHO report, about 17 million deaths are caused by cardiovascular and cerebrovascular diseases every year, accounting for 1 / 3 of various causes of death. Since 1958, the incidence of cardiovascular and cerebrovascular diseases in my country has increased by 4 times, accounting for 1 / 3 of the annual death population, and the current incidence trend is rising faster. Every 15 seconds, one Chinese person is diagnosed with cardiovascular and cerebrovascular diseases. Take lives, every 22 seconds there is a Chinese peo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P7/02
Inventor 蔡为荣张入飞
Owner ANHUI POLYTECHNIC UNIV
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