Tissue culture propagation method of Anemone vitifolia

Abstract

The invention discloses a tissue culture propagation method of Anemone vitifolia. Excellent effects of the high propagation speed, the stable hereditary character, the high propagation coefficient and vigorous growth of test-tube plantlets are realized mainly through steps of primary culture, propagation culture, rooting culture as well as plantlet exercise and transplantation, the germinating rate of calluses of the Anemone vitifolia is 96%, the propagation multiple is 7.3 times, the rooting rate is 98%, and the final survival rate is 96%.

Description

technical field [0001] The invention relates to a tissue culture propagation method suitable for wild cotton. Background technique [0002] Wild cotton (scientific name: Anemone vitifolia Buch.-Ham.) is a herbaceous plant with a plant height of 60-100 cm. Rhizome oblique, woody, leaf blade heart-shaped or broadly ovate. Scapes are strong, cymes 20-60 cm long, bracts 3, shaped like basal leaves, sepals 5, white or pinkish, obovate, filament-shaped; carpels about 400, aggregate fruit spherical, achenes thin handle. Flowers from July to October. Growing on grassy slopes, ditch sides or sparse forests. Distributed in China, Myanmar, Bhutan, Sikkim, Nepal and India. [0003] At present, no one has studied the technology of using tissue culture to breed wild cotton, the tissue culture system has not been established, the popularization of fine varieties has not been completed, and the contradiction between supply and demand of seedlings is increasing, which seriously affects ...

AI Technical Summary

Problems solved by technology

[0003] At present, no one has studied the technology of using tissue culture to breed wild cotton, the tissue culture system has not been established, the promotion and popularization of fine varieties has not been completed, and the contradiction between supply and demand of seedlings is increasing, which seriously affects the garden application and promotion of wild cotton