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Jingke 528 three-line mating hybrid seed production method

A hybrid seed production and sterile line technology, applied in the biological field, can solve problems such as long transfer cycle

Active Publication Date: 2015-03-11
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only using the traditional backcrossing transfer method usually requires backcrossing for 5-6 generations, and the transfer cycle is longer, which delays the application of sterile seed production technology in corn hybrids

Method used

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  • Jingke 528 three-line mating hybrid seed production method
  • Jingke 528 three-line mating hybrid seed production method
  • Jingke 528 three-line mating hybrid seed production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Embodiment 1, the breeding of male sterile line S Jing 724

[0077] 1. Determination of the type of male sterile line donor MD32 cytoplasmic male sterility

[0078] 1. Maize male sterile line MD32

[0079] Breeding process of maize male sterile line MD32: use the constructed X line population (based on X1132) to carry out "high and strict" line selection, and select excellent S5 high-generation lines and introduced hybrids to construct a new basic line selection population. Sterile plants were found in the selfed progeny, and the anthers of the tassels were not exposed, and sister plants with similar phenotypes in the same panicle row were selected for pair pollination. The hybrid offspring all showed complete sterility, and the plant phenotype tended to be basically the same. Then the material was named maize male sterile line MD32.

[0080] The corn sterile line MD32 is completely sterile: the anthers are not exposed;

[0081] The sterility of maize sterile line MD3...

Embodiment 2

[0125] Embodiment 2, the transfer of female parent 90110-2 male sterile line S 90110-2

[0126] Since 90110-2 (Corn Research Center of Beijing Academy of Agriculture and Forestry Sciences, the variety right application announcement number is CNA007813E) and Jing 724 are closely related, the CMS line S Jing 724 was selected as the donor material for the 90110-2 CMS transfer .

[0127] Using S Jing 724 as the female parent and 90110-2 as the male parent to cross F 1 Generation, F 1 The generation field showed complete sterility;

[0128] then F 1 Substitute female parent, continue to backcross with 90110-2 to get BC 1 generation groups.

[0129] From the planted 1500 BC 1 462 individual plant markers with complete male flower sterility and plant traits (plant height, ear height, axis color, number of tassel branches, ear traits and growth period, etc.) close to those of 90110-2 were selected from the generation population; BC 1 The DNA of a single plant leaf of the first ...

Embodiment 3

[0137] Example 3, Restoration Identification of Beijing 2437 to CMS Line S 90110-2

[0138] With the male sterile line S 90110-2 created in Example 2 as the female parent, Jing 2437 (recorded in the following documents: "Crop Magazine" 2009 04 issue of mid-maturity, high-yield and high-starch new corn variety Jingke 528, public can be obtained from Beijing Academy of Agriculture and Forestry Sciences) as the male parent, and the hybrid combination F 1 Generation, Harvest F 1 generation of seeds. The next year, plant F in Beijing, Hainan, Jilin and other fields 1 Generation, get F 1 Substitute plants. Observation identification F 1 Fertility restoration of tassels in generation plants showed that fertility returned to normal.

[0139] It shows that Jing 2437 is a strong restorer line of CMS line S 90110-2, which has full recovery ability.

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Abstract

The invention discloses a Jingke 528 three-line mating hybrid seed production method. The hybrid seed production method provided by the invention comprises the following steps: using a maize sterile line S 90110-2 as a sterile line, a maize inbred line 90110-2 as a maintainer line and a maize inbred line Jing 2437 as a restorer line to carry out three-line mating seed production to obtain a hybrid Jingke 528. Experimental results show that Jingke 528 three-line mating hybrid seed production is carried out by utilizing the bred sterile line S 90110-2, the maintainer line 90110-2 and the restorer line Jing 528, and prepared sterile hybrid Jingke 528 seeds are fundamentally the same as Jingke 528 seeds produced by using a conventional seed production method in the aspects of yield, resistance and other agronomic characters.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Jingke 528 three-line matching hybrid seed production method. Background technique [0002] Breeding and promotion of strong dominant hybrids is an important way to increase maize yield. The use of conventional seed production methods to prepare hybrids requires manual detasseling of the female parent, which not only consumes a lot of labor, increases the cost of seed production, but also has the potential risk of affecting seed quality due to untimely and incomplete detasseling. The use of male sterile lines for seed production is an effective method to ensure seed purity and increase maize yield. [0003] As early as the 1950s and 1960s, research on maize sterile seed production began at home and abroad. Cytoplasmic male sterility is the main type used in maize breeding because it is easy to realize the matching of sterile lines, maintainer lines and restorer lines. Cytoplasmi...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04C12Q1/68
Inventor 赵久然宋伟邢锦丰王元东段民孝刘春阁冯培煜张如养王凤格毛振武李瑞媛王乃顺王文广张莎莎
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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