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Reagent for detecting tumor marker tryptophan in urine and preparation method thereof

A technology for tumor markers and detection reagents, which is applied in the field of detection reagents and preparations for abnormal metabolites in the human body—tryptophan, which can solve the problems of low detection accuracy and achieve a simple detection process, improved specificity, and easy judgment Effect

Active Publication Date: 2015-03-04
无锡先君生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a reagent for detecting tumor marker tryptophan in urine and its preparation method, which are used to solve the problem of low detection accuracy

Method used

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  • Reagent for detecting tumor marker tryptophan in urine and preparation method thereof
  • Reagent for detecting tumor marker tryptophan in urine and preparation method thereof
  • Reagent for detecting tumor marker tryptophan in urine and preparation method thereof

Examples

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Effect test

Embodiment 1

[0027] The reagent for detecting the tumor marker tryptophan in the urine includes Hg 2+ , Hg 2 2+ , Ni 2+ , WO 4 2- , SO 4 2- , NO 3 - And SeO 3 2- Aqueous solution of which Hg 2+ The concentration is 0.21mol / L, Hg 2 2+ The concentration is 0.27mol / L, Ni 2+ The concentration is 6.3mmol / L, WO 4 2- The concentration is 1.2mmol / L, SeO 3 2- The concentration is 2.6mmol / L.

[0028] The preparation process is as follows:

[0029] 1) 1 part of commercially available concentrated sulfuric acid is diluted with water to 3 parts, cooled to room temperature for later use;

[0030] 2) 1 part of commercially available concentrated nitric acid is diluted with water to 3 parts, cooled to room temperature for later use;

[0031] 3) Preparation of solution A: weigh a certain amount of mercury sulfate and dissolve it in the sulfuric acid solution of step 1), in which the mercury ion content is 0.8 mol / L;

[0032] 4) Preparation of solution B: Weigh a certain amount of mercurous nitrate and dissolve it in...

Embodiment 2

[0038] The reagent for detecting the tumor marker tryptophan in the urine includes Hg 2+ , Hg 2 2+ , Ni 2+ , WO 4 2- , SO 4 2- , NO 3 - And SeO 3 2- Aqueous solution of which Hg 2+ The concentration is 0.15mol / L, Hg 2 2+ The concentration is 0.55mol / L, Ni 2+ The concentration is 2.0mmol / L, WO 4 2- The concentration is 2.4mmol / L, Se 3 2- The concentration is 1.1mmol / L.

[0039] The preparation process is as follows:

[0040] 1) 1 part of commercially available concentrated sulfuric acid is diluted with water to 4 parts, cooled to room temperature for later use;

[0041] 2) 1 part of commercially available concentrated nitric acid is diluted with water to 4 parts, cooled to room temperature for later use;

[0042] 3) Preparation of solution A: Weigh a certain amount of mercury sulfate and dissolve it in the sulfuric acid solution of step 1), in which the mercury ion content is 0.6 mol / L;

[0043] 4) Preparation of solution B: weigh a certain amount of mercurous nitrate and dissolve it in ...

Embodiment 3

[0049] The reagent for detecting the tumor marker tryptophan in the urine, including Hg 2+ , Hg 2 2+ , Ni 2+ , WO 4 2+- , SO 4 2- , NO 3 - And SeO 3 2- Aqueous solution of which Hg 2+ The concentration is 0.09mol / L, Hg 2 2+ The concentration is 0.85mol / L, Ni 2+ The concentration is 2.3mmol / L, WO 4 2- The concentration is 4.0mmol / L, SeO 3 2- The concentration is 3.4mmol / L.

[0050] The preparation process is as follows:

[0051] 1) 1 part of commercially available concentrated sulfuric acid is diluted with water to 5 parts, cooled to room temperature for later use;

[0052] 2) 1 part of commercially available concentrated nitric acid is diluted with water to 5 parts, cooled to room temperature for later use;

[0053] 3) Preparation of solution A: Weigh a certain amount of mercury sulfate and dissolve it in the sulfuric acid solution of step 1), where the content of mercury ions is 0.4 mol / L;

[0054] 4) Preparation of solution B: Weigh a certain amount of mercurous nitrate and dissolve i...

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Abstract

The invention belongs to urine detection reagents and particularly relates to a reagent for detecting an abnormal metabolite, namely tryptophan, in a human body and a preparation method of the reagent. The reagent for detecting a tumor marker, namely tryptophan, in urine comprises aqueous solutions of Hg<2+>, Hg2<2+>, Ni<2+>, WO4<2->, SO4<2->, NO3<-> and SeO3<2->. The preparation method comprises the following steps: preparing a sulfuric acid solution; preparing a nitric acid solution; preparing a solution A; preparing a solution B; preparing a solution C; preparing a solution D; preparing a solution E; mixing. According to the technical scheme provided by the invention, raw materials are easily obtained and are cheap, the preparation process is simple, the obtained reagent is stable in performance and has the characteristics of strong universality, high sensitivity, good specificity and the like when the reagent is applied to cancer detection, and the detection is simple in process, short in time and easy in judgment, so that the reagent is particularly applicable to the massive population check and the treatment effect check of cancer patients.

Description

Technical field [0001] The invention belongs to a urine detection reagent, and specifically relates to a reagent for detecting an abnormal metabolite in human body—tryptophan and a preparation method. Background technique [0002] Cancer mortality occupies the first place among all diseases and is the number one killer of human health. It has become a major cause of threat to human life and health. According to statistics, the number of cancer patients doubles every 25 years. Not only that, the number of deaths from cancer is also rapidly rising globally, and this number may increase to 13.2 million in 2030. Therefore, the prevention and treatment of cancer has become one of the key points of the global health strategy, and the key to the treatment of cancer lies in early detection, early diagnosis and early treatment. Image detection can often only detect mid- to late-stage tumor diseases, and it is difficult to achieve the purpose of early detection. It is expensive and compli...

Claims

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Application Information

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IPC IPC(8): G01N21/82
Inventor 刘东黎磊郑明彬
Owner 无锡先君生物医药有限公司
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