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Scnedesmus quadricauda for efficiently treating typical domestic sewage, and culture method and application thereof

A domestic sewage and a typical technology, applied in the field of high-efficiency treatment of typical domestic sewage and its cultivation field, can solve problems such as difficulty in achieving high efficiency, economy, low consumption, loss of nutrients, incomplete utilization of resources, etc., and achieve good oxidation Stabilizing performance, improving oil yield, increasing biodiesel yield

Inactive Publication Date: 2015-03-04
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional physical and chemical denitrification and phosphorus removal processes are difficult to meet the requirements of high efficiency, economy and low consumption in sewage treatment applications
Because algae have the advantages of high photosynthetic rate, fast growth rate, strong environmental adaptability, and fast adsorption, the use of algae to treat sewage can achieve efficient and low-cost removal of nutrients such as nitrogen and phosphorus. If algae are used, it can overcome traditional sewage treatment. The method is easy to cause secondary pollution, loss of potential nutrients, incomplete utilization of resources and other disadvantages

Method used

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  • Scnedesmus quadricauda for efficiently treating typical domestic sewage, and culture method and application thereof
  • Scnedesmus quadricauda for efficiently treating typical domestic sewage, and culture method and application thereof
  • Scnedesmus quadricauda for efficiently treating typical domestic sewage, and culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 4

[0028] Example 1 Separation and identification of Scenedesmus tetracerus SDEC-8:

[0029] 1. Sample collection

[0030] Water samples containing green algae were collected from a eutrophic lake in Quancheng Park, Jinan City, Shandong Province in July 2012.

[0031] 2. Isolation and purification of Scenedesmus tetracerus SDEC-8

[0032] Separation and purification step: Count the water samples in the botanical garden under the microscope by hemocytometer method, and dilute the density of the algae liquid to about 3000 / ml. On the ultra-clean test bench, pour the BG11 solid culture solution on the plate (it has been placed overnight, no bacteria growth), spray the algae liquid to be separated on the marked plate with a medical throat sprayer, then cover it, and put it on the plate. After cultivating in the incubator for more than ten days, isolated algae communities can occur on the medium, and the pure algae communities can be checked through a microscope, and then directly tr...

Embodiment 2 4

[0049] The BG11 culture medium of embodiment 2 Scenedesmus tetracerus SDEC-8

[0050] In order to make Scenedesmus tetracerus SDEC-8 grow faster and better, it was cultured on the basis of BG-11 medium.

[0051] (1) Medium used: The composition of BG11 medium is shown in Table 1. Before use, the pH was controlled at 7.1, sterilized at 120° C. for 30 minutes.

[0052] Table 1BG11 medium composition

[0053]

[0054]

[0055] The mother liquor is 1L of distilled water, sterilized after adjusting the pH to 7.1 with 1M NaOH or HCl, and ready for use.

[0056] (2) Culture conditions: Add the preserved algae seed solution to the volume ratio of 1:3 and add the culture solution, place it in a light incubator and cultivate it for 7 days, the temperature is 25±1°C, full light, the light intensity is 2000-3000Lux, Algae strains can be obtained.

Embodiment 3 4

[0057] Example 3 Optimization of Growth Conditions for Treatment of Typical Domestic Sewage by Scenedesmus tetracerus SDEC-8

[0058] In order to make Scenedesmus tetrascens SDEC-8 grow faster and better, after cultivating Scenedesmus tetrascens SDEC-8 according to the conditions and medium of embodiment 2, continue to cultivate on the basis of typical domestic sewage, and prepare high ((NH 4 ) 2 CO 3 291.1mg·L -1 , K 2 HPO 4 84.3mg·L -1 ), medium ((NH 4 ) 2 CO 3 137mg·L -1 , K 2 HPO 4 45mg·L -1 ), low ((NH 4 ) 2 CO 3 68.5mg·L -1 , K 2 HPO 4 22.5mg·L -1 ) artificial sewage with three different concentrations of nitrogen and phosphorus, and evaluate the pros and cons of the three culture conditions.

[0059] (1) Preparation of domestic sewage: According to the water quality of typical domestic sewage, the preparation of high ((NH 4 ) 2 CO 3 291.1mg·L -1 , K 2 HPO 4 84.3mg·L -1 ), medium ((NH 4 ) 2 CO 3 137mg·L -1 , K 2 HPO 4 45mg·L -1 ), low ((N...

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Abstract

The invention discloses a Scnedesmus quadricauda for efficiently treating typical domestic sewage, and a culture method and application thereof. Scnedesmus quadricauda SDEC-8 is cultured in a culture medium prepared according to the nitrogen and phosphorus concentrations of the typical domestic sewage, and is collected in the stabilization period. The result proves that the growth rate, oil content and biodiesel yield of the Scnedesmus quadricauda SDEC-8 in the prepared typical domestic sewage are respectively higher than those of the Scnedesmus quadricauda SDEC-8 cultured in a BG11 culture solution. When the total nitrogen and total phosphorus concentrations of the domestic sewage are respectively 40mg.L<-1> and 8mg.L<-1>, the oil content, fatty acid methyl ester content and fatty acid methyl ester yield of the Scnedesmus quadricauda SDEC-8 are maximum, and the biodiesel has the best properties. Besides, after the prepared typical domestic sewage is treated by the Scnedesmus quadricauda SDEC-8, the nitrogen and phosphorus can be efficiently removed, and the effluent water can reach Grade A (TN<15mg.L<-1>, and TP<0.5mg.L<-1>) in Discharge Standard of Pollutants for Municipal Sewage Treatment Plant. By using the typical domestic sewage to culture the Scnedesmus quadricauda SDEC-8, the nitrogen and phosphorus are efficiently removed, abundant microalgae biodiesel is efficiently obtained, and the microalgae culture cost is lowered.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, in particular to a Scenedesmus for efficiently treating typical domestic sewage and its cultivation method and application Background technique [0002] In recent years, due to the sharp increase in global consumption of fossil fuels and the reduction of petrochemical reserves, the worldwide energy crisis has been caused, coupled with the environmental problems caused by the emission of carbon dioxide from the combustion of fossil fuels, therefore, the search for a cheap green renewable energy has become It has become a scientific and social issue of widespread concern to governments and people all over the world. Studies have found that certain algae have the advantages of high oil content, easy cultivation, large yield per unit area, and no competition for land with agriculture. They are regarded as a new generation, and even the only biodiesel raw material that can replace fossil fuels....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C02F3/32C12P7/64C12R1/89
CPCC02F3/322C12N1/12C12N1/125C12R2001/89
Inventor 裴海燕宋明明胡文容韩琳张硕韩飞
Owner SHANDONG UNIV
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