Vegetable protein peptide stock solution as well as preparation method and application thereof
A technology of vegetable protein peptide and vegetable protein, applied in the field of cosmetics, can solve problems such as shortage and no specific research on mixing ratio.
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Embodiment 1
[0172] A kind of plant protein peptide stoste of the present embodiment, its active ingredient comprises the raw material of following weight:
[0173] Milk Thistle Extract 1g, Carnosine 0.01g, Palmitoyl Tripeptide-50.01g, Yeast Extract 0.1g.
[0174] The preparation method of above-mentioned a kind of vegetable protein peptide stoste, comprises the following steps:
[0175] (1) Mix 0.01g carnosine, 0.01g palmitoyl tripeptide-5, and 0.1g yeast extract, add 0.12g mannitol, dissolve in 0.12g phosphate buffer (pH6.8), and obtain liquid i;
[0176] (2) Take 1g of milk thistle extract, dissolve it in 25ml of hot acetone, keep it warm at 35°C, and obtain liquid Ⅱ;
[0177] (3) Mix liquid i with liquid ii, add 2g of lecithin, stir for 30min, vacuum concentrate to 1 / 5 of the original volume while shaking, add 25ml of n-hexane, filter after 20min, collect the upper liquid which is the plant of the present invention Protein peptide stock solution.
Embodiment 2
[0179] A kind of plant protein peptide stoste of the present embodiment, its active ingredient comprises the raw material of following weight:
[0180] Milk Thistle Extract 2g, Carnosine 0.02g, Palmitoyl Tripeptide-50.02g, Yeast Extract 0.2g.
[0181] The preparation method of above-mentioned a kind of vegetable protein peptide stoste, comprises the following steps:
[0182] (1) Mix 0.02g carnosine, 0.02g palmitoyl tripeptide-5, and 0.2g yeast extract, add 0.24g mannitol, and dissolve in 0.24g phosphate buffer (pH6.8) to obtain liquid i;
[0183] (2) Weigh 2g of milk thistle extract, dissolve it in 60ml of hot acetone, and keep it warm at 45°C to obtain liquid Ⅱ;
[0184] (3) Mix liquid i with liquid ii, add 6g lecithin, stir for 60min, vacuum concentrate to 1 / 6 of the original volume while shaking, add 60ml of n-hexane, filter after 25min, collect the upper layer liquid which is the plant of the present invention Protein peptide stock solution.
Embodiment 3
[0186] A kind of plant protein peptide stoste of the present embodiment, its active ingredient comprises the raw material of following weight:
[0187] Milk Thistle Extract 6g, Carnosine 0.12g, Palmitoyl Tripeptide-50.06g, Yeast Extract 1g.
[0188] The preparation method of above-mentioned a kind of vegetable protein peptide stoste, comprises the following steps:
[0189](1) Mix 0.12g carnosine, 0.06g palmitoyl tripeptide-5, and 1g yeast extract, add 1.18g mannitol, and dissolve in 1.18g phosphate buffer (pH6.8) to obtain liquid i;
[0190] (2) Weigh 6g of milk thistle extract, dissolve it in 180ml of hot acetone, and keep it warm at 45°C to obtain liquid Ⅱ;
[0191] (3) Mix liquid i with liquid ii, add 12g of lecithin, stir for 60min, vacuum concentrate to 1 / 5 of the original volume while shaking, add 180ml of n-hexane, filter after 25min, collect the upper liquid which is the plant of the present invention Protein peptide stock solution.
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