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Method for simultaneous determination of asperuloside acid and deacetylasperulosidicacid content in Noni juice by HPLC

A technology of deacetylpyrrodin and psyllidin, which is applied in the field of HPLC to simultaneously determine the content of psyllidin and deacetylpyrurinic acid in noni juice, can solve the problem of limiting the efficacy of noni juice Research on sexual components and other issues, to achieve the effect of high sensitivity, simple method and good reproducibility

Active Publication Date: 2015-02-25
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no HPLC method for the determination of psyllidin and deacetylated psyllidin in noni fruit juice, which limits the research on the functional components of noni fruit juice

Method used

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  • Method for simultaneous determination of asperuloside acid and deacetylasperulosidicacid content in Noni juice by HPLC
  • Method for simultaneous determination of asperuloside acid and deacetylasperulosidicacid content in Noni juice by HPLC
  • Method for simultaneous determination of asperuloside acid and deacetylasperulosidicacid content in Noni juice by HPLC

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Instruments and materials

[0020] 1.1 Instrument

[0021] Thermo UltiMate 3000 high performance liquid chromatography (VWD-3000 UV detector, Chromeleon 7 chromatographic workstation, Thermo Fisher Company), Shim-pack VP-ODS-C18 (250 mm×4.6 mm, 5 μm) chromatographic column; minispin centrifugal machine (eppendorf).

[0022] 1.2 Materials

[0023] The reference substance carurinic acid was purchased from Chengdu Pufeide Biotechnology Co., Ltd., deacetylated carurinic acid was purchased from Beijing Shengshikangpu Chemical Technology Research Institute, and acetonitrile and phosphoric acid were both chromatographically pure. Cisa Noni Juice is produced by Hainan Noni Biological Engineering Development Co., Ltd., and the product batches are 20120911, 20130618, 20131206 and 20140122.

[0024] Method and Results

[0025] 2.1 Selection of chromatographic conditions

[0026] Detector: Thermo VWD-3000; detection wavelength: 237nm; chromatographic column: Shim-pack V...

Embodiment 2

[0038] Methodological investigation

[0039] 1. Linear relationship investigation

[0040] Draw and mix 0.1, 0.2, 0.4, 0.6, 0.8 and 1.0 mL of the reference substance stock solution into a 10 mL volumetric flask, dilute with water to the mark, and shake well. Draw 10 μL of the above-mentioned reference substance solution and inject it into the liquid chromatograph, and perform linear regression on the mass concentration (x) with the peak area (y):

[0041] Pyloridinic acid: y = 4.405 x + 0.139, R2 = 0.9997, linear range 5-50 μg / mL.

[0042] Deacetylpyrrolidin: y = 5.030 x + 0.578, R2 = 0.9998, linear range 25-250 μg / mL.

[0043] 2. Instrument precision test

[0044] Take a portion of cisanoni juice (batch 20131206), inject 6 times continuously under the above-mentioned chromatographic conditions, record the concentration of pyridinic acid and deacetylated pyridinic acid, and the RSD values ​​are 0.44 % and 0.31 respectively %.

[0045] Table 3 Precision measurement resul...

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Abstract

The invention relates to a method for simultaneous determination of asperuloside acid and deacetylasperulosidicacid content in Noni juice by HPLC (high performance liquid chromatography). The technical proposal is as follows: respectively preparing a standard product solution and a sample solution, using the HPLC method for gradient elution for simultaneous determination of the asperuloside acid and deacetylasperulosidicacid content in the Noni juice. The method is simple, reliable, rapid, high in sensitivity, and good in reproducibility, can well separate asperuloside acid and deacetylasperulosidicacid in the Noni juice from interfering substances, can simultaneously determinate the asperuloside acid and deacetylasperulosidicacid content, and has important meaning to study on the effective components of the Noni juice.

Description

technical field [0001] The invention relates to a detection method for simultaneous determination of the content of pyridinic acid and deacetylated pyridinic acid in noni fruit juice by HPLC. Background technique [0002] Noni (Noni), scientific name Morinda officinalis ( Morinda citrifolia Linn . ), in May 2010, the Chinese Ministry of Health officially approved noni pulp as a new resource food. Noni fruit juice has a wide range of nutritional and medicinal values. It has the functions of anti-tumor, anti-oxidation, enhancing immunity, lowering blood fat and protecting cardiovascular, and has preventive and therapeutic effects on many diseases. [0003] Kurosidic acid and deacetylyluric acid are iridoid substances, which are important active ingredients in noni fruit juice. They have anti-tumor, anti-virus, anti-oxidation, anti-distortion and other activities, and are closely related to human health. It has gradually become a research hotspot. However, there is currentl...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 程池陈建国张露李金霞洪启恩谭望桥
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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