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Chitosanase and method for producing chitosan oligosaccharide by using same

A technology of chitosanase and glycanase, applied in the field of chitosanase and chitosan oligosaccharide production, can solve the problems of limited reaction conditions and low thermal stability, and achieve the effect of high heat resistance

Inactive Publication Date: 2015-02-25
ZTH BEIJING TECH DEV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, among the currently developed enzymes for the production of chitosan oligosaccharides, most of the chitosanases have low thermal stability and can only remain stable at lower temperatures below 50°C. The degree of polymerisation of chitosanases is limited in terms of reaction conditions

Method used

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  • Chitosanase and method for producing chitosan oligosaccharide by using same
  • Chitosanase and method for producing chitosan oligosaccharide by using same

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Embodiment Construction

[0035] In order to better understand a kind of chitosanase according to the scheme of the present invention and the method for using the polycanase to produce chitosan oligosaccharides, a preferred embodiment of the squid block grading device of the present invention will be further elaborated below.

[0036] In order to produce chitosan oligosaccharides with a higher content of oligosaccharides of more than 6 sugars by enzymatic method, the inventors of the present invention have worked hard to find that there is a heat-resistant strain of Bacillus AMI09 isolated from the soil on the south coast of Gyeongnam, South Korea. High-performance chitosanase, utilizing the heat-resistant chitosanase to be able to produce chitosan oligosaccharides with high 6-saccharide content under high-temperature optimum reaction conditions, thereby completing the present invention. Thus, the object of the present invention is to provide a method for producing high-polymerization-degree chitosan ol...

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Abstract

The invention relates to a chitosanase and a method for producing chitosan oligosaccharide by using same. The chitosanase is prepared according to the following steps: inoculating Bacillus sp. AMI09 which is separated from soil on the south coast of Kyongnam in South Korea and can produce chitosan oligosaccharide with high polymerization degree into an LB culture solution, then carrying out shaking culture to obtain a culture solution for inoculation, performing centrifugal separation of a great number of culture solution which is cultured in an MS culture medium to obtain supernatant, concentrating and washing the supernatant, performing ion exchange chromatography and gel filtration chromatography, and finally dialyzing with deionized water to obtain chitosanase. The heat-resistant chitosanase prepared by the strain can stably produce chitosan oligosaccharide with high polymerization degree and high hexanose content under the reaction condition of 60 DEG C.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a chitosanase and a method for producing chitosan oligosaccharides using the polycanase. Background technique [0002] Chitosan is a polysaccharide composed of D-glucosamine linked by β-1,4 bonds, and is generally made by deacetylating chitin. This kind of chitosan can be used in various production fields as a polymer substance with a molecular weight of hundreds of thousands of daltons, but its application in production is limited due to the problems of high viscosity and insolubility in neutral or alkaline water. application. In order to solve this technical problem, people have tried the method of producing water-soluble chitosan oligosaccharide by decomposing chitosan. Through trying to know that when the prepared chitosan oligosaccharide has a polymerization degree of 6 sugars or more, it has a greater ability to enhance human immunity, anti-tumor and antibacterial effect...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12P19/26C12R1/07
CPCC12N9/2402C12P19/26C12Y302/01132
Inventor 李炜朴哲
Owner ZTH BEIJING TECH DEV
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